Rabbit GDI1 Polyclonal Antibody | anti-GDI1 antibody

GDI1 Polyclonal Antibody

Cat. Num. AAA3005873

• Gene Names: GDI1; 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GDI1; Polyclonal Antibody; GDI1 Polyclonal Antibody; Rab GDP dissociation inhibitor alpha; Rab GDI alpha; Rab GDIalpha; RabGDIalpha; Guanosine diphosphate dissociation inhibitor 1; GDI-1; Oligophrenin-2; Protein XAP-4; GDIL; OPHN2; RABGDIA; XAP4; anti-GDI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GDI1 polyclonal antibody detects endogenous levels of GDI1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 447

• Applicable Applications for anti-GDI1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 402-447 of Human GDI1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GDI1 polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysateLane2:Mouse liver tissue lysateLane3:Rat liver tissue lysate)

Related Product Information for anti-GDI1 antibody

Rab proteins, a family of Ras-related small GTP-binding proteins, play a key role in regulating intracellular vesicle trafficking. Rab GDP dissociation inhibitor (Rab GDI or GDI3) forms a soluble complex with Rab proteins and thereby prevents the exchange of GDP for GTP. In mammals, there exist two major isoforms, Rab GDI alpha (also known as XAP-4) and Rab GDI beta. While the mammalian Rab GDI beta-genes are ubiquitously expressed, the Rab GDI alpha genes are predominantly brain-specific. Since it is expressed predominantly in neural and sensory tissues, Rab GDI alpha may serve a specific function in neural signalmtransmission. The gene sequences for the Rab GDI proteins are extremely conserved in evolution, with substantial homology preserved across three eukaryotic kingdoms.

NCBI and Uniprot Product Information

• NCBI GI #: 49457546
• NCBI GeneID: 2664
• UniProt Accession #: P31150
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: GDI1, partial
• NCBI Official Synonym Full Names: GDP dissociation inhibitor 1
• NCBI Official Symbol: GDI1
• NCBI Official Synonym Symbols: 1A; GDIL; MRX41; MRX48; OPHN2; XAP-4; RABGD1A; RABGDIA
• NCBI Protein Information: rab GDP dissociation inhibitor alpha
• UniProt Protein Name: Rab GDP dissociation inhibitor alpha
• Protein Family: Guanosine nucleotide diphosphate dissociation inhibitor
• UniProt Gene Name: GDI1
• UniProt Synonym Gene Names: GDIL; OPHN2; RABGDIA; XAP4; Rab GDI alpha; GDI-1
• UniProt Entry Name: GDIA_HUMAN

NCBI Description

GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific cognitive disability. [provided by RefSeq, Jul 2008]

Uniprot Description

GDI1: a GDP dissociation inhibitor protein expressed primarily in neural and sensory tissues. GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the Rab family, small GTP-binding proteins of the Ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from Rab proteins and release GDP from membrane-bound Rabs. Mutations have been linked to X-linked nonspecific mental retardation.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: Golgi apparatus; neuron projection; protein complex; cytoplasm; midbody; cytosol

Molecular Function: protein binding; GDP-dissociation inhibitor activity; Rab GDP-dissociation inhibitor activity; Rab GTPase binding; GTPase activator activity

Biological Process: protein transport; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; negative regulation of axonogenesis; Rab protein signal transduction; response to calcium ion; signal transduction; positive regulation of GTPase activity

Disease: Mental Retardation, X-linked 41