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Western Blot (WB) (GDF6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the GDF6 antibody detected the GDF6 protein (arrow).)

Rabbit anti-Mouse GDF6 Polyclonal Antibody | anti-GDF6 antibody

GDF6, NT (GDF6, GDF16, Growth/differentiation factor 6, Growth/differentiation factor 16) (MaxLight 750)

Gene Names
GDF6; KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; MCOP4; SCDO4; MCOPCB6
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
GDF6; Polyclonal Antibody; NT (GDF6; GDF16; Growth/differentiation factor 6; Growth/differentiation factor 16) (MaxLight 750); Growth/differentiation factor 16; anti-GDF6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-GDF6 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
GDF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human GDF6.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GDF6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the GDF6 antibody detected the GDF6 protein (arrow).)

Western Blot (WB) (GDF6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the GDF6 antibody detected the GDF6 protein (arrow).)

Immunohistochemistry (IHC)

(GDF6 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GDF6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (GDF6 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GDF6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GDF6 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (GDF6 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-GDF6 antibody
This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene result in colobomata, which are congenital abnormalities in ocular development, and in Klippel-Feil syndrome (KFS), which is a congenital disorder of spinal segmentation.
Product Categories/Family for anti-GDF6 antibody
References
Gonzalez-Rodriguez, J., et al. Br J Ophthalmol 94(8):1100-1104(2010). Mikic, B., et al. J. Orthop. Res. 27(12):1603-1611(2009). Asai-Coakwell, M., et al. Hum. Mol. Genet. 18(6):1110-1121(2009). Zhang, X., et al. Mol. Vis. 15, 2911-2918 (2009): Shen, B., et al. Int. J. Biol. Sci. 5(2):192-200(2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,662 Da
NCBI Official Full Name
growth/differentiation factor 6
NCBI Official Synonym Full Names
growth differentiation factor 6
NCBI Official Symbol
GDF6
NCBI Official Synonym Symbols
KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; MCOP4; SCDO4; MCOPCB6
NCBI Protein Information
growth/differentiation factor 6; GDF-6; Klippel-Feil syndrome; Klip-Feil malformation; Klippel-Feil malformation; growth/differentiation factor 16
UniProt Protein Name
Growth/differentiation factor 6
UniProt Gene Name
GDF6
UniProt Synonym Gene Names
GDF16; GDF-6
UniProt Entry Name
GDF6_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene result in colobomata, which are congenital abnormalities in ocular development, and in Klippel-Feil syndrome (KFS), which is a congenital disorder of spinal segmentation. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF6: Required for normal formation of bones and joints in the limbs, skull, and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Defects in GDF6 are the cause of Klippel-Feil syndrome type 1 (KFS1). A skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Deafness is a well- known feature of KFS and may be of sensorineural, conductive, or mixed type. A chromosomal aberration involving GDF6 has been found in a patient with Klippel-Feil syndrome (KFS). Paracentric inv(8)(q22;2q23.3). Defects in GDF6 are the cause of microphthalmia isolated type 4 (MCOP4). A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide; Cytokine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: extracellular space

Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; regulation of MAPKKK cascade; activin receptor signaling pathway; positive regulation of neuron differentiation; cell development; growth

Disease: Klippel-feil Syndrome 1, Autosomal Dominant; Microphthalmia, Isolated 4; Leber Congenital Amaurosis 17; Microphthalmia, Isolated, With Coloboma 6

Research Articles on GDF6

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Product Notes

The GDF6 gdf6 (Catalog #AAA6301828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GDF6, NT (GDF6, GDF16, Growth/differentiation factor 6, Growth/differentiation factor 16) (MaxLight 750) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GDF6 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GDF6 gdf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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