Rabbit anti-Human, Mouse GCLM Polyclonal Antibody | anti-GCLM antibody

GCLM, CT (GCLM, GLCLR, Glutamate-cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate-cysteine ligase modifier subunit) (Biotin)

Cat. Num. AAA6301656

• Gene Names: GCLM; GLCLR
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GCLM; Polyclonal Antibody; CT (GCLM; GLCLR; Glutamate-cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate-cysteine ligase modifier subunit) (Biotin); Glutamate--cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; anti-GCLM antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GCLM antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GCLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245~274 amino acids from the C-terminal region of human GCLM.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GCLM Antibody (C-term) in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GCLM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCLM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GCLM Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of GCLM (arrow) using rabbit polyclonal GCLM Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCLM gene.)

Western Blot (WB)

(Western blot analysis of GCLM (arrow) using rabbit polyclonal GCLM Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCLM gene.)

Related Product Information for anti-GCLM antibody

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Product Categories/Family for anti-GCLM antibody

Antibodies; Amino Acids

References

Moyer, A.M., et.al., Cancer Epidemiol. Biomarkers Prev. 19 (3), 811-821 (2010). Engstrom, K.S., et.al., Mutat. Res. 683 (1-2), 98-105 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4504011
• NCBI GeneID: 2730
• NCBI Accession #: NP_002052.1
• NCBI GenBank Nucleotide #: NM_002061.2
• UniProt Accession #: P48507; Q6FHC1; Q9NPX9; Q9NU74; A8K334; D3DT45
• Molecular Weight: 30,727 Da
• NCBI Official Full Name: glutamate--cysteine ligase regulatory subunit
• NCBI Official Synonym Full Names: glutamate-cysteine ligase, modifier subunit
• NCBI Official Symbol: GCLM
• NCBI Official Synonym Symbols: GLCLR
• NCBI Protein Information: glutamate--cysteine ligase regulatory subunit; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit
• UniProt Protein Name: Glutamate--cysteine ligase regulatory subunit
• Protein Family: Glutamate--cysteine ligase regulatory
• UniProt Gene Name: GCLM
• UniProt Synonym Gene Names: GLCLR
• UniProt Entry Name: GSH0_HUMAN

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Protein type: Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: protein heterodimerization activity; glutamate-cysteine ligase activity; enzyme regulator activity; glutamate-cysteine ligase catalytic subunit binding

Biological Process: response to drug; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; glutamate metabolic process; sulfur amino acid metabolic process; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; negative regulation of neuron apoptosis; regulation of mitochondrial depolarization; regulation of blood vessel size; positive regulation of glutamate-cysteine ligase activity

Disease: Myocardial Infarction, Susceptibility To