Rabbit anti-Human GBL Polyclonal Antibody | anti-GBL antibody

GBL, CT (MLST8, GBL, LST8, Target of rapamycin complex subunit LST8, G protein beta subunit-like, Mammalian lethal with SEC13 protein 8) (FITC)

Cat. Num. AAA6301448

• Gene Names: MLST8; GBL; LST8; POP3; WAT1; GbetaL; MGC111011; MLST8
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GBL; Polyclonal Antibody; CT (MLST8; LST8; Target of rapamycin complex subunit LST8; G protein beta subunit-like; Mammalian lethal with SEC13 protein 8) (FITC); MLST8; Mammalian lethal with SEC13 protein 8; anti-GBL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GBL antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GBL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human GBL.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GBL Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the GBL antibody detected the GBL protein (arrow).)

Related Product Information for anti-GBL antibody

Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Product Categories/Family for anti-GBL antibody

Antibodies; Proteins

References

Ali, S.M., et al. J. Biol. Chem. 280(20):19445-19448(2005). Inoki, K., et al. Microbiol. Mol. Biol. Rev. 69(1):79-100(2005). Sarbassov, D.D., et al. Science 307(5712):1098-1101(2005). Jacinto, E., et al. Nat. Cell Biol. 6(11):1122-1128(2004). Kim, D.H., et al. Mol. Cell 11(4):895-904(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 84626578
• NCBI GeneID: 64223
• UniProt Accession #: Q9BVC4; Q5M800; Q86Y18; Q8WUI5; Q9HA66; Q9UJV6; B3KMM4; B4DY00
• NCBI Official Full Name: target of rapamycin complex subunit LST8 [Homo sapiens]
• NCBI Official Synonym Full Names: MTOR associated protein, LST8 homolog (S. cerevisiae)
• NCBI Official Symbol: MLST8
• NCBI Official Synonym Symbols: GBL; LST8; POP3; WAT1; GbetaL; MGC111011; MLST8
• UniProt Protein Name: Target of rapamycin complex subunit LST8
• UniProt Gene Name: MLST8
• UniProt Synonym Gene Names: GBL; LST8; TORC subunit LST8; Gable; Protein GbetaL; mLST8
• UniProt Entry Name: LST8_HUMAN

Uniprot Description

LST8: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum- induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12- rapamycin. Interacts directly with MTOR and RPTOR. Interacts with RHEB. Broadly expressed, with highest levels in skeletal muscle, heart and kidney. Belongs to the WD repeat LST8 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of actin filament polymerization; nerve growth factor receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway