Rabbit anti-Human GATA6 Polyclonal Antibody | anti-GATA6 antibody

GATA6, Phosphorylated (Tyr271) (GATA Binding Factor 6, GATA Binding Protein 6, Transcription Factor GATA-6) (FITC)

Cat. Num. AAA6477583

• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GATA6; Polyclonal Antibody; Phosphorylated (Tyr271) (GATA Binding Factor 6; GATA Binding Protein 6; Transcription Factor GATA-6) (FITC); anti-GATA6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GATA6 when phosphorylated at Tyr271.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GATA6 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr271 of human GATA6 (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-GATA6-pY271 Phospho-specific Pab (RB15308) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-GATA6 antibody

Antibodies; Transcription Factors

References

1. Kwei, K.A., PLoS Genet. 4(5), E1000081 (2008). 2. Ghatnekar, A., Biochim. Biophys. Acta 1779(3), 145-151 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 40288197
• NCBI GeneID: 2627
• NCBI Accession #: NP_005248
• NCBI GenBank Nucleotide #: NM_005257.4
• UniProt Accession #: Q92908; P78327; B0YJ17
• Molecular Weight: 45,386 Da
• NCBI Official Full Name: transcription factor GATA-6
• NCBI Official Synonym Full Names: GATA binding protein 6
• NCBI Official Symbol: GATA6
• NCBI Protein Information: transcription factor GATA-6
• UniProt Protein Name: Transcription factor GATA-6
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA6
• UniProt Entry Name: GATA6_HUMAN

NCBI Description

This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012]

Uniprot Description

GATA6: Transcriptional activator that regulates SEMA3C and PLXNA2. Thought to be important for regulating terminal differentiation and/or proliferation. Interacts with LMCD1. Expressed in heart, gut and gut-derived tissues. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q11.1-q11.2

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; zinc ion binding; chromatin binding; transcription factor activity; transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; response to drug; cardiac muscle hypertrophy; endodermal cell fate determination; organ formation; in utero embryonic development; male gonad development; negative regulation of transcription from RNA polymerase II promoter; liver development; cardiac muscle cell differentiation; negative regulation of transforming growth factor-beta1 production; negative regulation of transforming growth factor-beta2 production; positive regulation of angiogenesis; response to estrogen stimulus; phospholipid metabolic process; positive regulation of cardioblast differentiation; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; blood coagulation; tube morphogenesis; negative regulation of apoptosis; positive regulation of cardiac muscle cell proliferation

Disease: Conotruncal Heart Malformations; Atrial Septal Defect 9; Heart Defects, Congenital, And Other Congenital Anomalies; Tetralogy Of Fallot; Atrioventricular Septal Defect 5