Rabbit anti-Human GATA2 Polyclonal Antibody | anti-GATA2 antibody

GATA2, CT (Endothelial Transcription Factor GATA-2, GATA-binding Protein 2, GATA2) (MaxLight 405)

Cat. Num. AAA6477552

• Gene Names: GATA2; DCML; IMD21; NFE1B; MONOMAC
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GATA2; Polyclonal Antibody; CT (Endothelial Transcription Factor GATA-2; GATA-binding Protein 2; GATA2) (MaxLight 405); anti-GATA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GATA2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-GATA2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GATA2 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GATA2 Antibody (C-term) in K562 cell line lysates (35ug/lane). GATA2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using GATA2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of GATA2 (arrow) using rabbit polyclonal GATA2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GATA2 gene.)

Product Categories/Family for anti-GATA2 antibody

Antibodies; Transcription Factors

References

1. Pan, X., et.al., J. Biochem. 127(1), 105-112 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 20070352
• NCBI GeneID: 2624
• NCBI Accession #: NP_116027
• NCBI GenBank Nucleotide #: NM_032638
• UniProt Accession #: P23769
• Molecular Weight: 50kDa
• NCBI Official Full Name: endothelial transcription factor GATA-2 isoform 1
• NCBI Official Synonym Full Names: GATA binding protein 2
• NCBI Official Symbol: GATA2
• NCBI Official Synonym Symbols: DCML; IMD21; NFE1B; MONOMAC
• NCBI Protein Information: endothelial transcription factor GATA-2
• UniProt Protein Name: Endothelial transcription factor GATA-2
• Protein Family: GATA-binding factor
• UniProt Gene Name: GATA2
• UniProt Entry Name: GATA2_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; protein complex

Molecular Function: protein binding; zinc ion binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: embryonic placenta development; somatic stem cell maintenance; cell maturation; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; cell differentiation in hindbrain; positive regulation of phagocytosis; positive regulation of megakaryocyte differentiation; central nervous system neuron development; inner ear morphogenesis; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; regulation of histone acetylation; negative regulation of fat cell differentiation; phagocytosis; positive regulation of angiogenesis; negative regulation of macrophage differentiation; homeostasis of number of cells within a tissue; pituitary gland development; ventral spinal cord interneuron differentiation; commitment of a neuronal cell to a specific type of neuron in the forebrain; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; urogenital system development

Disease: Lymphedema, Primary, With Myelodysplasia; Immunodeficiency 21; Myelodysplastic Syndrome