Western Blot (WB)
(Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GATA2 (Phospho-Ser401) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit anti-Human GATA2 Polyclonal Antibody | anti-GATA2 antibody
GATA2 (Phospho-Ser401) Antibody
Gene Names
GATA2; DCML; IMD21; NFE1B; MONOMAC
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
GATA2; Polyclonal Antibody; GATA2 (Phospho-Ser401) Antibody; Endothelial transcription factor GATA-2; GATA binding protein 2; anti-GATA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
GATA2 (Phospho-Ser401) Antibody detects endogenous levels of GATA2 only when phosphorylated at Ser401.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
466
Applicable Applications for anti-GATA2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:1000
ELISA: 1:1000
Target Modification
Phospho
Modification Sites
H:S401
Immunogen
The antiserum was produced against synthesized peptide derived from human GATA2 around the phosphorylation site of Ser401.
Immunogen Range
367-416
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GATA2 (Phospho-Ser401) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GATA2 (Phospho-Ser401) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50 kDa
NCBI Official Full Name
endothelial transcription factor GATA-2 isoform 1
NCBI Official Synonym Full Names
GATA binding protein 2
NCBI Official Symbol
GATA2
NCBI Official Synonym Symbols
DCML; IMD21; NFE1B; MONOMAC
NCBI Protein Information
endothelial transcription factor GATA-2
UniProt Protein Name
Endothelial transcription factor GATA-2
Protein Family
UniProt Gene Name
GATA2
NCBI Description
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
Uniprot Description
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
Research Articles on GATA2
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Product Notes
The GATA2 gata2 (Catalog #AAA9502761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GATA2 (Phospho-Ser401) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the GATA2 gata2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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