Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells untreated or treated with Anisomycin using Rabbit anti GATA1 (pSer310) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
Rabbit GATA1 Polyclonal Antibody | anti-GATA1 antibody
Rabbit anti GATA1 (pSer310)
Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Applications
Western Blot
Purity
Purified whole serum by affinity chromatography.
Synonyms
GATA1; Polyclonal Antibody; Rabbit anti GATA1 (pSer310); GATA1 (pSer310) Antibody; anti-GATA1 antibody
Host
Rabbit
Reactivity
Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified whole serum by affinity chromatography.
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Sequence Length
413
Applicable Applications for anti-GATA1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 310 of human GATA1
Preperation
Antiserum to GATA1 (pSer310) was raised by repeated immunization of rabbits with highly purified antigen.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells untreated or treated with Anisomycin using Rabbit anti GATA1 (pSer310) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells untreated or treated with Anisomycin using Rabbit anti GATA1 (pSer310) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
Related Product Information for anti-GATA1 antibody
Rabbit anti GATA1 (pSer310) antibody recognizes GATA1 when phosphorylated at serine 310. GATA1 is a member of the GATA transcription factor family and plays a significant role in the maturation of blood cells such as red blood cells, mast cells and eosinophils. Phosphorylation of GATA1 at serine 310 occurs via activation of AKT. Phosphorylation of GATA1 at serine 310 plays a role in normal and pathogenic erythopoietis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,232 Da
NCBI Official Full Name
Erythroid transcription factor
NCBI Official Synonym Full Names
GATA binding protein 1
NCBI Official Symbol
GATA1
NCBI Official Synonym Symbols
GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
NCBI Protein Information
erythroid transcription factor
UniProt Protein Name
Erythroid transcription factor
Protein Family
UniProt Gene Name
GATA1
UniProt Synonym Gene Names
ERYF1; GF1; GATA-1; GF-1
NCBI Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).
Research Articles on GATA1
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Product Notes
The GATA1 gata1 (Catalog #AAA225260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti GATA1 (pSer310) reacts with Human, Mouse (Expected from Sequence), Rat (Expected from Sequence) and may cross-react with other species as described in the data sheet. AAA Biotech's GATA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the GATA1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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