Western Blot (WB)
(GATA-4 antibody (pAb) tested by Western blot. Western Blot: Nuclear extract (20 ug) of F9 cells (retinoic acid treated mouse teratocarcinoma F9 stem cell line,000.)
Rabbit anti-Human, Mouse GATA-4 Polyclonal Antibody | anti-GATA-4 antibody
GATA-4 antibody (pAb)
Gene Names
GATA4; TOF; ASD2; VSD1; TACHD
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purified
Synonyms
GATA-4; Polyclonal Antibody; GATA-4 antibody (pAb); ASD2; VSD1; TACHD; anti-GATA-4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
0.66ug/ul (varies by lot)
Applicable Applications for anti-GATA-4 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
WB: 1:500-1:2,000 dilution
Immunogen
This GATA-4 antibody was raised against a peptide derived from the C-terminus of human GATA-4.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(GATA-4 antibody (pAb) tested by Western blot. Western Blot: Nuclear extract (20 ug) of F9 cells (retinoic acid treated mouse teratocarcinoma F9 stem cell line,000.)
Western Blot (WB)
(GATA-4 antibody (pAb) tested by Western blot. Western Blot: Nuclear extract (20 ug) of F9 cells (retinoic acid treated mouse teratocarcinoma F9 stem cell line,000.)
Related Product Information for anti-GATA-4 antibody
Short Description: Our GATA-4 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human and Mouse samples.
Background: GATA-4 is a zinc finger protein and part of the GATA family of sequence specific DNA binding transcription factors that recognize a consensus DNA sequence containing GATA. GATA-4 is involved in the development of several mesodermal and endodermal structures. GATA-4 mutant mice exhibit severe heart malformations. Defects in GATA-4 are the cause of atrial septal defect type 2. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. GATA-4 also plays a role in regulating gene expression in cardiac and smooth muscle cell types.
Background: GATA-4 is a zinc finger protein and part of the GATA family of sequence specific DNA binding transcription factors that recognize a consensus DNA sequence containing GATA. GATA-4 is involved in the development of several mesodermal and endodermal structures. GATA-4 mutant mice exhibit severe heart malformations. Defects in GATA-4 are the cause of atrial septal defect type 2. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. GATA-4 also plays a role in regulating gene expression in cardiac and smooth muscle cell types.
Product Categories/Family for anti-GATA-4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,665 Da
NCBI Official Full Name
transcription factor GATA-4
NCBI Official Synonym Full Names
GATA binding protein 4
NCBI Official Symbol
GATA4
NCBI Official Synonym Symbols
TOF; ASD2; VSD1; TACHD
NCBI Protein Information
transcription factor GATA-4; GATA-binding factor 4
UniProt Protein Name
Transcription factor GATA-4
UniProt Gene Name
GATA4
UniProt Entry Name
GATA4_HUMAN
Similar Products
Product Notes
The GATA-4 gata4 (Catalog #AAA388624) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GATA-4 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GATA-4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution. Researchers should empirically determine the suitability of the GATA-4 gata4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA-4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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