Rabbit anti-Human GART Polyclonal Antibody | anti-GART antibody

GART (Trifunctional Purine Biosynthetic Protein Adenosine-3, PGFT, PRGS, MGC47764) (HRP)

Cat. Num. AAA6379432

• Gene Names: GART; AIRS; GARS; PAIS; PGFT; PRGS; GARTF
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GART; Polyclonal Antibody; GART (Trifunctional Purine Biosynthetic Protein Adenosine-3; PGFT; PRGS; MGC47764) (HRP); anti-GART antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GART.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 433

• Applicable Applications for anti-GART antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human GART, aa1-433 (NP_780294.1).
• Immunogen Sequence: MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKDVGFRAIAFLQQPR
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GART rabbit polyclonal antibody. Western Blot analysis of GART expression in human placenta.)

Western Blot (WB)

(Western Blot analysis of GART expression in transfected 293T cell line by GART rabbit polyclonal antibody. Lane 1: GART transfected lysate (46kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-GART antibody

The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Product Categories/Family for anti-GART antibody

Antibodies; Enzymes; Synthetase

NCBI and Uniprot Product Information

• NCBI GI #: 28416899
• NCBI GeneID: 2618
• NCBI Accession #: NP_780294.1
• NCBI GenBank Nucleotide #: NM_175085.1
• UniProt Accession #: P22102
• NCBI Official Full Name: trifunctional purine biosynthetic protein adenosine-3 isoform 2
• NCBI Official Synonym Full Names: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
• NCBI Official Symbol: GART
• NCBI Official Synonym Symbols: AIRS; GARS; PAIS; PGFT; PRGS; GARTF
• NCBI Protein Information: trifunctional purine biosynthetic protein adenosine-3
• UniProt Protein Name: Trifunctional purine biosynthetic protein adenosine-3
• UniProt Gene Name: GART
• UniProt Synonym Gene Names: PGFT; PRGS; GARS; AIRS; GART
• UniProt Entry Name: PUR2_HUMAN

NCBI Description

The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

GART: a highly conserved trifunctional enzyme. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. Inhibition of the de novo purine synthesis by folate analogs blocks the p53-dependent G1 checkpoint in human carcinoma cell lines. Two splice variant isoforms have been described.

Protein type: Methyltransferase; Ligase; EC 6.3.3.1; Nucleotide Metabolism - purine; EC 2.1.2.2; EC 6.3.4.13; Cofactor and Vitamin Metabolism - one carbon pool by folate; Mitochondrial; Enzyme, cellular metabolism

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: cytosol

Molecular Function: methyltransferase activity; metal ion binding; phosphoribosylamine-glycine ligase activity; phosphoribosylformylglycinamidine cyclo-ligase activity; phosphoribosylglycinamide formyltransferase activity; ATP binding

Biological Process: methylation; response to organic substance; purine ribonucleoside monophosphate biosynthetic process; glycine metabolic process; tetrahydrofolate biosynthetic process; response to inorganic substance; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; cerebellum development; cerebral cortex development; purine base metabolic process; 'de novo' IMP biosynthetic process