Rabbit GANP Polyclonal Antibody | anti-GANP antibody

GANP Rabbit Polyclonal

Cat. Num. AAA769572

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: GANP; Polyclonal Antibody; GANP Rabbit Polyclonal; anti-GANP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 1980

• Applicable Applications for anti-GANP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Immunogen: Minichromosome maintenance complex component 3 associated protein
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS769572 (GANP antibody) at dilution of 1:50)

Western Blot (WB)

(mouse brain tissue were subjected to SDS PAGE followed by western blot with MBS769572 (GANP antibody) at dilution of 1:300)

NCBI and Uniprot Product Information

• NCBI GI #: 5734402
• UniProt Accession #: O60318; Q2M3C1; Q6PJP6; Q9BSY5; Q9UMT4; C9JL56
• Molecular Weight: 80,292 Da
• NCBI Official Full Name: GANP protein
• UniProt Protein Name: Germinal-center associated nuclear protein
• Protein Family: Putative arabinogalactan oligomer transport system permease protein
• UniProt Gene Name: MCM3AP
• UniProt Synonym Gene Names: GANP; KIAA0572; MAP80; GANP; MCM3AP
• UniProt Entry Name: GANP_HUMAN

Uniprot Description

GANP: is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3¿ region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-¿B, and p53 consensus transcription factor binding sites are located. In contrast, the GANP promoter region contains consensus transcription factor binding sites for E2F, E-box, BSAP, and PU.1. MCM3AP interacts with MCM3, which it can acetylate weakly in vivo. GANP is required for efficient mRNA nuclear export: GANP depletion results in the nuclear accumulation of poly(A)+RNA. It interacts via its N-terminal FG repeat domain with the mRNA export factor NXF1. GANP plays a role in the immune response: antibodies from mice deficient for GANP have reduced affinities against T-cell dependent antigens and a lower frequency of variable region somatic mutation. GANP expression is markedly decreased in malignant glioma (MG) with a poor prognosis, consistently lower than that observed in anaplastic astrocytomas. Significant decreases in GANP expression are associated with hyperploidy and chromosomal instability

Protein type: Acetyltransferase; Karyopherin

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; cytosol; nuclear membrane; nuclear pore; nucleoplasm; nucleus

Molecular Function: DNA binding; nucleotide binding; transferase activity, transferring acyl groups

Biological Process: DNA replication; immune system process; mRNA transport; protein import into nucleus