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Western Blot (WB) (Western Blot analysis in mouse spleen tissue lysates (35ug/lane) uaing MBS642650. GALNT5 (arrow) was detected using MBS642650.)

Rabbit anti-Human, Mouse GALNT5 Polyclonal Antibody | anti-GALNT5 antibody

GALNT5, NT (GALNT5, Polypeptide N-acetylgalactosaminyltransferase 5, Polypeptide GalNAc transferase 5, Protein-UDP acetylgalactosaminyltransferase 5, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5)

Gene Names
GALNT5; GALNACT5; GALNAC-T5
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
GALNT5; Polyclonal Antibody; NT (GALNT5; Polypeptide N-acetylgalactosaminyltransferase 5; Polypeptide GalNAc transferase 5; Protein-UDP acetylgalactosaminyltransferase 5; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5); Anti -GALNT5; anti-GALNT5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-GALNT5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
GALNT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31~61 amino acids from the N-terminal region of human GALNT5.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse spleen tissue lysates (35ug/lane) uaing MBS642650. GALNT5 (arrow) was detected using MBS642650.)

Western Blot (WB) (Western Blot analysis in mouse spleen tissue lysates (35ug/lane) uaing MBS642650. GALNT5 (arrow) was detected using MBS642650.)

Western Blot (WB)

(Western Blot analysis of lysates from A431, K562 cell line (from left to right, 35ug/lane), using MBS642650 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Western Blot (WB) (Western Blot analysis of lysates from A431, K562 cell line (from left to right, 35ug/lane), using MBS642650 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human normal lung tissue using MBS642650 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642650 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human normal lung tissue using MBS642650 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642650 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MBS642650 (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using MBS642650 (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-GALNT5 antibody
GALNT5 can catalyze the initial reaction in O-linked oligosaccharide biosynthesis,the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT5 has activity toward EA2 peptide substrate, but it has a weak activity toward Muc2 or Muc1b substrates.
Product Categories/Family for anti-GALNT5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
106,266 Da
NCBI Official Full Name
GALNT5 protein
NCBI Official Synonym Full Names
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 (GalNAc-T5)
NCBI Official Symbol
GALNT5
NCBI Official Synonym Symbols
GALNACT5; GALNAC-T5
NCBI Protein Information
polypeptide N-acetylgalactosaminyltransferase 5; pp-GaNTase 5; polypeptide GalNAc transferase 5; protein-UDP acetylgalactosaminyltransferase 5; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5
UniProt Protein Name
Polypeptide N-acetylgalactosaminyltransferase 5
UniProt Gene Name
GALNT5
UniProt Synonym Gene Names
GalNAc-T5
UniProt Entry Name
GALT5_HUMAN

Uniprot Description

GALNT5: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward EA2 peptide substrate, but has a weak activity toward Muc2 or Muc1b substrates. Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.41

Chromosomal Location of Human Ortholog: 2q24.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: polypeptide N-acetylgalactosaminyltransferase activity; metal ion binding; carbohydrate binding

Biological Process: protein amino acid O-linked glycosylation; glycosaminoglycan biosynthetic process; cellular protein metabolic process; O-glycan processing; post-translational protein modification

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Product Notes

The GALNT5 galnt5 (Catalog #AAA642650) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GALNT5, NT (GALNT5, Polypeptide N-acetylgalactosaminyltransferase 5, Polypeptide GalNAc transferase 5, Protein-UDP acetylgalactosaminyltransferase 5, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the GALNT5 galnt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALNT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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