Rabbit anti-Human, Mouse GALNT3 Polyclonal Antibody | anti-GALNT3 antibody

GALNT3 Polyclonal Antibody

Cat. Num. AAA3011615

• Gene Names: GALNT3; HHS; HFTC; HFTC1; GalNAc-T3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GALNT3; Polyclonal Antibody; GALNT3 Polyclonal Antibody; Polypeptide N-acetylgalactosaminyltransferase 3; Polypeptide GalNAc transferase 3; GalNAc-T3; pp-GaNTase 3; Protein-UDP acetylgalactosaminyltransferase 3; UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase 3; anti-GALNT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GALNT3 polyclonal antibody detects endogenous levels of GALNT3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 633

• Applicable Applications for anti-GALNT3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human GALNT3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using GALNT3 antibody.)

Related Product Information for anti-GALNT3 antibody

The UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosaminyl) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in the golgi apparatus. GalNAc-T3, also known as GALNT3, HHS or HFTC, is a 633 amino acid single-pass type II membrane protein that localizes to the golgi and contains one ricin B-type lectin domain. Expressed at high levels in kidney, testis, skin and pancreas, GalNAc-T3 uses manganese and calcium as cofactors to catalyze the first reaction in O-linked oligosaccharide biosynthesis, namely the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on a target protein. Defects in the gene encoding GalNAc-T3 are the cause of hyperphosphatemic familial tumoral calcinosis (HFTC) and hyperostosis-hyperphosphatemia syndrome (HHS). GalNAc-T3 is overexpressed in carcinoma tissue, suggesting a role in tumor development and metastasis.

NCBI and Uniprot Product Information

• NCBI GI #: 153266878
• NCBI GeneID: 2591
• NCBI Accession #: NP_004473.2
• NCBI GenBank Nucleotide #: NP_004473.2
• UniProt Accession #: Q14435
• Molecular Weight: ~ 73kDa
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 3
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 3
• NCBI Official Symbol: GALNT3
• NCBI Official Synonym Symbols: HHS; HFTC; HFTC1; GalNAc-T3
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 3
• UniProt Gene Name: GALNT3
• UniProt Synonym Gene Names: GalNAc-T3; pp-GaNTase 3

NCBI Description

This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq, Jul 2008]

Uniprot Description

GALNT3: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has activity toward HIV envelope glycoprotein gp120, EA2, Muc2 and Muc5. Probably glycosylates fibronectin in vivo. Plays a central role in phosphate homeostasis. Mutations cause cause familial tumoral calcinosis, a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Overexpressed in many differentiated carcinomas, suggesting that it may serve as a marker of tumor differentiation. Two alternatively spliced human isoforms have been described.

Protein type: EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: extracellular exosome; Golgi apparatus; Golgi membrane; integral component of membrane; membrane; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; carbohydrate binding; manganese ion binding; polypeptide N-acetylgalactosaminyltransferase activity

Biological Process: carbohydrate metabolic process; fibroblast growth factor receptor signaling pathway; O-glycan processing; protein O-linked glycosylation via serine; protein O-linked glycosylation via threonine

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial