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Western Blot (WB) (Western blot analysis of GALNT10 in rat brain tissue lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.)

Rabbit GALNT10 Polyclonal Antibody | anti-GALNT10 antibody

GALNT10 Antibody

Gene Names
GALNT10; GALNACT10; PPGALNACT10; PPGANTASE10
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
GALNT10 Antibody is affinity chromatography purified via peptide column.
Synonyms
GALNT10; Polyclonal Antibody; GALNT10 Antibody; GALNACT10; PPGALNACT10; PPGANTASE10; Polypeptide N-acetylgalactosaminyltransferase 10; Polypeptide GalNAc transferase 10; GalNAc-T10; UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10); anti-GALNT10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
GALNT10 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
603
Applicable Applications for anti-GALNT10 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
GALNT10 antibody can be used for detection of GALNT10 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
GALNT10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human GALNT10.
Buffer
GALNT10 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
GALNT10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of GALNT10 in rat brain tissue lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.)

Western Blot (WB) (Western blot analysis of GALNT10 in rat brain tissue lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of GALNT10 in human brain tissue with GALNT10 antibody at 2.5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of GALNT10 in human brain tissue with GALNT10 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of GALNT10 in human brain tissue with GALNT10 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of GALNT10 in human brain tissue with GALNT10 antibody at 20 μg/mL.)
Related Product Information for anti-GALNT10 antibody
GALNT10 Antibody: Protein glycosylation is an important biological process that is carried out by a large family of glycosyltransferases that catalyze the synthesis of oligosaccharides and glycoconjugates. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Polypeptide galactoaminyltransferase 10 (GALNT10) belongs to the polypeptide N-acetylgalactosaminyl-transferase (pp-GalNAc-T) protein family. Following expression in insect cells, recombinant GALNT10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both non-glycosylated and glycosylated peptide substrates. GALNT10 mRNA is highly expressed in several distinct hypothalamic, thalamic, and amygdaloid nuclei in mouse brain. At least four isoforms of GALNT10 are known to exist.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,833 Da
NCBI Official Full Name
polypeptide N-acetylgalactosaminyltransferase 10
NCBI Official Synonym Full Names
polypeptide N-acetylgalactosaminyltransferase 10
NCBI Official Symbol
GALNT10
NCBI Official Synonym Symbols
GALNACT10; PPGALNACT10; PPGANTASE10
NCBI Protein Information
polypeptide N-acetylgalactosaminyltransferase 10; GalNAc transferase 10; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10; UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)
UniProt Protein Name
Polypeptide N-acetylgalactosaminyltransferase 10
UniProt Gene Name
GALNT10
UniProt Synonym Gene Names
GalNAc-T10; pp-GaNTase 10
UniProt Entry Name
GLT10_HUMAN

NCBI Description

This gene encodes a member of the GalNAc polypeptide N-acetylgalactosaminyltransferases. These enzymes catalyze the first step in the synthesis of mucin-type oligosaccharides. These proteins transfer GalNAc from UDP-GalNAc to either serine or threonine residues of polypeptide acceptors. The protein encoded by this locus may have increased catalytic activity toward glycosylated peptides compared to activity toward non-glycosylated peptides.[provided by RefSeq, Apr 2010]

Uniprot Description

GALNT10: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward Muc5Ac and EA2 peptide substrates. Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.41; Transferase

Chromosomal Location of Human Ortholog: 5q33.2

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: polypeptide N-acetylgalactosaminyltransferase activity; metal ion binding; carbohydrate binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification

Research Articles on GALNT10

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Product Notes

The GALNT10 galnt10 (Catalog #AAA150879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GALNT10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). GALNT10 antibody can be used for detection of GALNT10 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the GALNT10 galnt10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALNT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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