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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Galectin 9 (GAL9) Polyclonal Antibody | anti-GAL9 antibody

Polyclonal Antibody to Galectin 9 (GAL9)

Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Galectin 9 (GAL9); Polyclonal Antibody; Polyclonal Antibody to Galectin 9 (GAL9); anti-GAL9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GAL9. It has been selected for its ability to recognize GAL9 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-FFTSIPN GFYPSKSINI SGVVLPDAKR FHINLRCGGD IAFHLNPRFN EKVVVRNTQI NNSWGPEERS LPGRMPFNRG QSFSVWILCE GHCFKVAVDG QHICEYYHRL KNLPDINTLE VAGDIQLTHV QT
Sequence Length
323
Applicable Applications for anti-GAL9 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GAL9 (Phe194~Thr322) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040936
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded spleen tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded spleen tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Pancreas Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Pancreas Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,317 Da
NCBI Official Full Name
galectin-9
NCBI Official Synonym Full Names
galectin 9
NCBI Official Symbol
Lgals9
NCBI Protein Information
galectin-9
UniProt Protein Name
Galectin-9
Protein Family
UniProt Gene Name
Lgals9
UniProt Synonym Gene Names
Gal-9; UAT

NCBI Description

a highly selective urate transporter/channel [RGD, Feb 2006]

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: cytoplasm; cytosol; extracellular region; extracellular space; intracellular; nucleus

Molecular Function: carbohydrate binding; disaccharide binding; enzyme binding; galactoside binding; protein serine/threonine kinase activator activity; receptor binding; signal transducer activity

Biological Process: female pregnancy; inflammatory response; ion transport; maintenance of protein location; maternal process involved in female pregnancy; natural killer cell tolerance induction; negative regulation of activated T cell proliferation; negative regulation of chemokine production; negative regulation of inflammatory response; negative regulation of interferon-gamma production; negative regulation of mast cell degranulation; negative regulation of natural killer cell activation; negative regulation of natural killer cell degranulation; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of tumor necrosis factor production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation during immune response; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of innate immune response; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-1 production; positive regulation of interleukin-4 production; positive regulation of macrophage activation; positive regulation of NF-kappaB import into nucleus; positive regulation of NF-kappaB transcription factor activity; positive regulation of oxidoreductase activity; positive regulation of regulatory T cell differentiation; positive regulation of tumor necrosis factor production; positive regulation of virion penetration into host cell; regulation of interleukin-4 production; regulation of interleukin-5 production; regulation of natural killer cell differentiation; regulation of T cell differentiation in the thymus; response to lipopolysaccharide; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway

Research Articles on GAL9

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Product Notes

The GAL9 lgals9 (Catalog #AAA2005654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Galectin 9 (GAL9) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin 9 (GAL9) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GAL9 lgals9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-FFTSIP N GFYPSKSINI SGVVLPDAKR FHINLRCGGD IAFHLNPRFN EKVVVRNTQI NNSWGPEERS LPGRMPFNRG QSFSVWILCE GHCFKVAVDG QHICEYYHRL KNLPDINTLE VAGDIQLTHV QT. It is sometimes possible for the material contained within the vial of "Galectin 9 (GAL9), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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