Rabbit anti-Human, Mouse GAK Polyclonal Antibody | anti-GAK antibody

GAK (Q129) Polyclonal Antibody

Cat. Num. AAA3001798

• Gene Names: GAK; DNAJ26; DNAJC26
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GAK; Polyclonal Antibody; GAK (Q129) Polyclonal Antibody; Cyclin-G-associated kinase; anti-GAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GAK (Q129) polyclonal antibody detects endogenous levels of GAK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1311

• Applicable Applications for anti-GAK antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human GAK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of GAK (Q129) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-GAK antibody

Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. Cyclin E and Cdk2 interact during the G1 to S transition. Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor). Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. A serine/threonine kinase, designated GAK for cyclin G associated kinase, has been identified. GAK has been shown to bind directly to cyclin G and to co-immunoprecipitate with Cdk5, which also associates with cyclin G.

NCBI and Uniprot Product Information

• NCBI GI #: 157384971
• NCBI GeneID: 2580
• NCBI Accession #: NP_005246.2
• NCBI GenBank Nucleotide #: NP_005246.2
• UniProt Accession #: O14976
• Molecular Weight: ~ 143kDa
• NCBI Official Full Name: cyclin-G-associated kinase isoform 1
• NCBI Official Synonym Full Names: cyclin G associated kinase
• NCBI Official Symbol: GAK
• NCBI Official Synonym Symbols: DNAJ26; DNAJC26
• NCBI Protein Information: cyclin-G-associated kinase
• UniProt Protein Name: Cyclin-G-associated kinase
• Protein Family: Cyclin-G-associated kinase
• UniProt Gene Name: GAK
• UniProt Entry Name: GAK_HUMAN

NCBI Description

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Other group; NAK family

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi apparatus; focal adhesion; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: endoplasmic reticulum organization and biogenesis; epidermal cell differentiation; forebrain morphogenesis; cell cycle; cell development; Golgi organization and biogenesis; protein amino acid phosphorylation