Goat GADD45gamma Polyclonal Antibody | anti-GADD45G antibody
Goat anti-GADD45gamma (aa 18 to 28) Antibody
Gene Names
GADD45G; CR6; DDIT2; GRP17; GADD45gamma
Reactivity
Expected from sequence similarity: Human, Mouse, Rat
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GADD45gamma; Polyclonal Antibody; Goat anti-GADD45gamma (aa 18 to 28) Antibody; Goat Anti-GADD45gamma (aa 123 to 133) Antibody; growth arrest and DNA-damage-inducible; gamma; CR6; DDIT2; GRP17; GADD45-gamma; gadd-related protein; 17 kD; growth arrest and DNA-damage-inducible gamma; gamma antibody; CR6 antibody; DDIT2 antibody; GADD45gamma antibody; GRP17 antibody; GADD45-gamma antibody; 17 kD antibody; growth arrest and DNA-damage-inducible gamma antibody; GADD45gamma (aa 18 to 28); anti-GADD45G antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RMQGAGKALHE
Sequence Length
159
Applicable Applications for anti-GADD45G antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave an approx 23kDa band in Human Testis and Thyroid Gland lysates and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_006696.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 23kDa band in Human Testis and Thyroid Gland lysates and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_006696.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-RMQGAGKALHE, from the internal region of the protein sequence according to NP_006696.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,121 Da
NCBI Official Full Name
growth arrest and DNA damage-inducible protein GADD45 gamma
NCBI Official Synonym Full Names
growth arrest and DNA damage inducible gamma
NCBI Official Symbol
GADD45G
NCBI Official Synonym Symbols
CR6; DDIT2; GRP17; GADD45gamma
NCBI Protein Information
growth arrest and DNA damage-inducible protein GADD45 gamma
UniProt Protein Name
Growth arrest and DNA damage-inducible protein GADD45 gamma
Protein Family
UniProt Gene Name
GADD45G
UniProt Synonym Gene Names
CR6; DDIT2; DDIT-2
UniProt Entry Name
GA45G_HUMAN
NCBI Description
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]
Uniprot Description
GADD45G: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK. Belongs to the GADD45 family.
Protein type: Nuclear receptor co-regulator; Apoptosis; DNA repair, damage
Chromosomal Location of Human Ortholog: 9q22.1-q22.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding
Biological Process: activation of MAPKKK activity; apoptosis; cell differentiation; multicellular organismal development; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cell cycle; response to stress
Research Articles on GADD45G
Similar Products
Product Notes
The GADD45G gadd45g (Catalog #AAA421580) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GADD45gamma (aa 18 to 28) Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GADD45gamma can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave an approx 23kDa band in Human Testis and Thyroid Gland lysates and in lysates of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_006696.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the GADD45G gadd45g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RMQGAGKALH E. It is sometimes possible for the material contained within the vial of "GADD45gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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