Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreas cancer using MBS769525 (GADD34 antibody) at dilution of 1:100)
Rabbit GADD34 Polyclonal Antibody | anti-GADD34 antibody
GADD34 Rabbit Polyclonal
Gene Names
Gadd34; CG3825; dGADD34; Dm-GADD34; DmelCG3825; dPPP1R15; gadd34; GADD34
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>=95% as determined by SDS-PAGEImmunogen Affinity Purified
Synonyms
GADD34; Polyclonal Antibody; GADD34 Rabbit Polyclonal; growth arrest and DNA damage-inducible protein 34; PPP1R15A; anti-GADD34 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Immunogen Affinity Purified
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Sequence Length
317
Applicable Applications for anti-GADD34 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB 1:200-1 :1000
IHC 1:20-1:200
IF 1:20-1:200
IHC 1:20-1:200
IF 1:20-1:200
Immunogen
Protein phosphatase 1, regulatory (inhibitor) subunit 15A
Preparation and Storage
Store at -20°C (Avoid repeated freeze / thaw cycles.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreas cancer using MBS769525 (GADD34 antibody) at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreas cancer using MBS769525 (GADD34 antibody) at dilution of 1:100)
Immunofluorescence (IF)
(Immunofluorescent analysis of PC-3 cells using MBS769525 (GADD34 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of PC-3 cells using MBS769525 (GADD34 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L))
Western Blot (WB)
(K-562 cells were subjected to SDS PAGE followed by western blot with MBS769525 (GADD34 antibody) at dilution of 1:600)
Western Blot (WB)
(K-562 cells were subjected to SDS PAGE followed by western blot with MBS769525 (GADD34 antibody) at dilution of 1:600)
Related Product Information for anti-GADD34 antibody
GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed MW: 100 kDa
NCBI Official Full Name
Gadd34
NCBI Official Symbol
Gadd34
NCBI Official Synonym Symbols
CG3825; dGADD34; Dm-GADD34; DmelCG3825; dPPP1R15; gadd34; GADD34
NCBI Protein Information
CG3825 gene product from transcript CG3825-RA
Research Articles on GADD34
Similar Products
Product Notes
The GADD34 (Catalog #AAA769525) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GADD34 Rabbit Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GADD34 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB 1:200-1 :1000IHC 1:20-1:200
IF 1:20-1:200. Researchers should empirically determine the suitability of the GADD34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GADD34, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
