Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5% SDS PAGE gel and GAD67 antibody at a dilution of 1:1000)
Rabbit GAD67 Polyclonal Antibody | anti-GAD67 antibody
GAD67 Antibody
Gene Names
GAD1; GAD; SCP; CPSQ1
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
GAD67; Polyclonal Antibody; GAD67 Antibody; Affinity Purified Rabbit Polyclonal GAD67 Antibody; Polyclonal GAD67 antibody; Anti-GAD67 antibody; GAD1 antibody; FLJ45882 antibody; GAD-67; GAD-67 antibody; glutamate decarboxylase 1 antibody; glutamate decarboxylase 67 kDa isoform antibody; GAD 67; GAD 67 antibody; SCP antibody; GAD antibody; anti-GAD67 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
594
Applicable Applications for anti-GAD67 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:3000
ICC: 1:100-1:500
WB: 1:500-1:3000
Immunogen
GAD67 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5% SDS PAGE gel and GAD67 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5% SDS PAGE gel and GAD67 antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GAD67 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GAD67 antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and GAD67 antibody at a dilution of 1:500)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and GAD67 antibody at a dilution of 1:500)
Related Product Information for anti-GAD67 antibody
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.
Product Categories/Family for anti-GAD67 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
67 kDa
NCBI Official Full Name
glutamic acid decarboxylase 67 kda form
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
NCBI Protein Information
glutamate decarboxylase 1
NCBI Description
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
Research Articles on GAD67
Similar Products
Product Notes
The GAD67 (Catalog #AAA5311832) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GAD67 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the GAD67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAD67, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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