Rabbit anti-Human GAD65 Polyclonal Antibody | anti-GAD65, B78 antibody

Anti-GAD65, B78 Antibody

Cat. Num. AAA415808

• Gene Names: GAD2; GAD65
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: GAD65; Polyclonal Antibody; Anti-GAD65; B78 Antibody; Anti-GAD65 Antibody; anti-GAD65; B78 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2824

• Applicable Applications for anti-GAD65, B78 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA titer: 1:40,000-1:80,000; Western blot analysis: 1:200-500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

References

(1)Oak,S., Gilliam,L.K., Landin-Olsson,M., et al.The lack of anti-idiotypic antibodies, not the presence of the corresponding autoantibodies to glutamate decarboxylase, defines type 1 diabetes. Proc. Natl. Acad. Sci. U.S.A. 105 (14), 5471-5476 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 197276618
• NCBI GeneID: 2572
• NCBI Accession #: NP_000809.1
• NCBI GenBank Nucleotide #: NM_000818
• Molecular Weight: 65,411 Da
• NCBI Official Full Name: Homo sapiens glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• NCBI Official Symbol: GAD2
• NCBI Official Synonym Symbols: GAD65
• NCBI Protein Information: glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform
• UniProt Protein Name: Glutamate decarboxylase 2
• UniProt Gene Name: GAD2
• UniProt Entry Name: DCE2_HUMAN

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Uniprot Description

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Lyase; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - taurine and hypotaurine; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cell junction; cytosol

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process