Rabbit anti-Mouse GAD65 Polyclonal Antibody | anti-GAD2 antibody
Anti-GAD65 Antibody, Rabbit Polyclonal
Gene Names
Gad2; GAD65; Gad-2; GAD(65); 6330404F12Rik
Reactivity
Mouse
Applications
ELISA
Purity
Protein A & Antigen Affinity
Synonyms
GAD65; Polyclonal Antibody; Anti-GAD65 Antibody; Rabbit Polyclonal; GAD65/GAD2/GAD-2 Antibody; Rabbit PAb; Antigen Affinity Purified; glutamate decarboxylase 2 (pancreatic islets and brain; 65kDa); Anti-6330404F12Rik Antibody; Anti-GAD(65) Antibody; Anti-Gad-2 Antibody; anti-GAD2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse GAD65/GAD2/GAD-2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GAD2 antibody
ELISA (EIA)
Application Notes
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse GAD65/GAD2/GAD-2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse GAD65/GAD2/GAD-2 (rM GAD65/GAD2/GAD-2). GAD65/GAD2/GAD-2 specific IgG was purified by Mouse GAD65/GAD2/GAD-2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Related Product Information for anti-GAD2 antibody
Glutamate decarboxylase 2, also known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid decarboxylase, GAD2 and GAD65, is a member of thegroup II decarboxylase family. GAD2 is identified as a major autoantigen in insulin-dependent diabetes. GAD2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. GABA is synthesized in brain by two isoforms of glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand. Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of GAD2 increased ethanol palatability and intake and slightly reduced the severity of ethanol-induced withdrawal.
Product Categories/Family for anti-GAD2 antibody
References
Karlsen A.E., et al., (1991), Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10. Proc. Natl. Acad. Sci. U.S.A. 88:8337-8341.
Bu D.-F., et al., (1992), Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.Proc. Natl. Acad. Sci. U.S.A. 89:2115-2119.
Bu D.-F., et al., (1994), The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derive from a common ancestral GAD.Genomics 21:222-228.
Bu D.-F., et al., (1992), Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.Proc. Natl. Acad. Sci. U.S.A. 89:2115-2119.
Bu D.-F., et al., (1994), The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derive from a common ancestral GAD.Genomics 21:222-228.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,224 Da
NCBI Official Full Name
glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamic acid decarboxylase 2
NCBI Official Symbol
Gad2
NCBI Official Synonym Symbols
GAD65; Gad-2; GAD(65); 6330404F12Rik
NCBI Protein Information
glutamate decarboxylase 2
UniProt Protein Name
Glutamate decarboxylase 2
Protein Family
UniProt Gene Name
Gad2
UniProt Synonym Gene Names
Gad65; GAD-65
UniProt Entry Name
DCE2_MOUSE
Uniprot Description
GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Lyase
Cellular Component: axon; cell junction; cytoplasm; cytoplasmic vesicle; cytosol; Golgi apparatus; membrane; perinuclear region of cytoplasm; plasma membrane; synapse; synaptic vesicle membrane
Molecular Function: carboxy-lyase activity; catalytic activity; glutamate binding; glutamate decarboxylase activity; lyase activity; protein heterodimerization activity; pyridoxal phosphate binding
Biological Process: carboxylic acid metabolic process; glutamate decarboxylation to succinate; neurotransmitter biosynthetic process
Research Articles on GAD2
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Product Notes
The GAD2 gad2 (Catalog #AAA8111881) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GAD65 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GAD65 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the GAD2 gad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAD65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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