Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
Rabbit anti-Human; Mouse GABARAPL1 Polyclonal Antibody | anti-GABARAPL1 antibody
GABARAPL1 Antibody: ATTO 390
Gene Names
GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
Reactivity
Human; Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Peptide Affinity Purified
Synonyms
GABARAPL1; Polyclonal Antibody; GABARAPL1 Antibody: ATTO 390; Rabbit Anti-Human GABARAPL1 Polyclonal; APG8 like Antibody; GEC-1 Antibody; ATG8 Antibody; Glandular epithelial cell protein 1 Antibody; ATG8B Antibody; GEC1 Antibody; GBRL1_HUMAN Antibody; Gamma-aminobutyric acid receptor-associated protein-like 1 Antibody; GABARAPL1 a Antibody; ATG8L Antibody; APG8L Antibody; GABA(A) receptor-associated protein-like 1 Antibody; Early estrogen regulated protein Antibody; Early estrogen-regulated protein Antibody; GABA(A) receptor associated protein like 1 Antibody; Gamma aminobutyric acid receptor associated protein like 1 Antibody; GEC 1 Antibody; GABARAPL1 Antibody; GABA Antibody; anti-GABARAPL1 antibody
Host
Rabbit
Reactivity
Human; Mouse
Clonality
Polyclonal
Specificity
Detects ~18 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
117
Applicable Applications for anti-GABARAPL1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000
ICC/IF: 1:100
ICC/IF: 1:100
Target
GABARAPL1
Immunogen
Synthetic peptide from the N-terminal of Human GABARAPL1 (aa. 5-16)
Immunogen Species
Human
Cellular Localization
Cytoplasm; Cytoskeleton; Cytoplasmic Vesicle Membrane; Lipid-Anchor; Endoplasmic Reticulum; Golgi Apparatus; Cytoplasmic Vesicle; Autophagosome
Conjugate
ATTO 390
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
Product Categories/Family for anti-GABARAPL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,008 Da
NCBI Official Full Name
gamma-aminobutyric acid receptor-associated protein-like 1
NCBI Official Synonym Full Names
GABA type A receptor associated protein like 1
NCBI Official Symbol
GABARAPL1
NCBI Official Synonym Symbols
ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
NCBI Protein Information
gamma-aminobutyric acid receptor-associated protein-like 1
UniProt Protein Name
Gamma-aminobutyric acid receptor-associated protein-like 1
Protein Family
UniProt Gene Name
GABARAPL1
UniProt Synonym Gene Names
GEC1; GEC-1
Uniprot Description
Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Research Articles on GABARAPL1
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Product Notes
The GABARAPL1 gabarapl1 (Catalog #AAA809473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GABARAPL1 Antibody: ATTO 390 reacts with Human; Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GABARAPL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ICC/IF: 1:100. Researchers should empirically determine the suitability of the GABARAPL1 gabarapl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABARAPL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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