Western Blot (WB)
(Western blot analysis of extract from rat brain and mouse brain tissue and C6 cells using GABA A Receptor a3 Antibody)
Rabbit GABA A Polyclonal Antibody | anti-GABA A antibody
GABA A Receptor a 3 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Synonyms
GABA A; Polyclonal Antibody; GABA A Receptor a 3 Antibody; Gamma-aminobutyric acid receptor subunit alpha-3; Gabra3; anti-GABA A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total GABA A Receptor a3 protein.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
493
Applicable Applications for anti-GABA A antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:1000
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around aa. 33~37(R-R-Q-E-P)derived from Rat GABA A Receptor a3.
Target Name
GABA A Receptor a3
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Western Blot (WB)
(Western blot analysis of extract from rat brain and mouse brain tissue and C6 cells using GABA A Receptor a3 Antibody)
Western Blot (WB)
(Western blot analysis of extract from rat brain and mouse brain tissue and C6 cells using GABA A Receptor a3 Antibody)
Related Product Information for anti-GABA A antibody
GABA (g-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
Product Categories/Family for anti-GABA A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
51kd
NCBI Official Full Name
Gamma-aminobutyric acid receptor subunit alpha-3
NCBI Official Synonym Full Names
gamma-aminobutyric acid (GABA) A receptor, alpha 3
NCBI Official Symbol
Gabra3
NCBI Protein Information
gamma-aminobutyric acid receptor subunit alpha-3
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit alpha-3
UniProt Gene Name
Gabra3
UniProt Synonym Gene Names
Gabra-3
UniProt Entry Name
GBRA3_RAT
NCBI Description
may mediate inhibitory GABA responses; may play a role in synaptic transmission in the visual cortex [RGD, Feb 2006]
Uniprot Description
GABRA3: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel
Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel
Cellular Component: postsynaptic membrane; integral to membrane; plasma membrane; synapse; cell junction
Molecular Function: chloride channel activity; protein binding; GABA-A receptor activity; extracellular ligand-gated ion channel activity
Biological Process: synaptic transmission; chloride transport; gamma-aminobutyric acid signaling pathway
Research Articles on GABA A
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Product Notes
The GABA A gabra3 (Catalog #AAA9402376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GABA A Receptor a 3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GABA A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:1000. Researchers should empirically determine the suitability of the GABA A gabra3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABA A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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