Rabbit Gab2 Polyclonal Antibody | anti-GAB2 antibody

Phospho-Gab2 (Tyr643) Antibody

Cat. Num. AAA9611963

• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: Gab2; Polyclonal Antibody; Phospho-Gab2 (Tyr643) Antibody; GAB 2; GAB2_HUMAN; Grb 2 associated binder 2; GRB 2 associated binding protein 2; Grb2 associated binder 2; GRB2 associated binder 2 pp100; GRB2 associated binding protein 2; GRB2-associated binder 2; GRB2-associated-binding protein 2; Growth factor receptor bound protein 2 associated protein 2; Growth factor receptor bound protein 2-associated protein 2; KIAA0571; p97; PH domain containing adaptor molecule p97; pp100; anti-GAB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-Gab2 (Tyr643) Antibody detects endogenous levels of Gab2 only when phosphorylated at tyrosine 643.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 638

• Applicable Applications for anti-GAB2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 643(V-D-Y(p)-V-Q) derived from Human Gab2.
• Fragment: Fab fragment
• Conjugation: Unconjugated
• Epitope: Phospho Tyr643

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, using Phospho-Gab2 (Tyr643) Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-GAB2 antibody

Function: Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

Post Translational Modifications: Phosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.Dephosphorylated by PTPN11.

Subcellular Location: Cytoplasm. Cell membrane.

Subunit Structure: Interacts with SHC1; may mediate interaction with receptors (By similarity). Interacts with SYK (By similarity). Interacts with PI-3 kinase. Interacts with GRB2 (via SH3 2 domain). Interacts (phosphorylated) with PTPN11. Interacts with TNFRSF11A (via cytoplasmic domain). Interacts (phosphorylated) with 14-3-3 family proteins SFN, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ; prevents interaction with GRB2 and attenuates GAB2 signaling. Interacts with HCK.

Similarity: The SH3-binding motifs mediate interaction with SHC1 and GRB2.The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding.Belongs to the GAB family.

NCBI and Uniprot Product Information

• NCBI GI #: 6912460
• NCBI GeneID: 9846
• NCBI Accession #: NP_036428.1
• NCBI GenBank Nucleotide #: NP_036428.1
• UniProt Accession #: Q9UQC2
• Molecular Weight: Observed Molecular Weight: (Observed)74kD.; Predicted Molecular Weight: (Calculated)74kDa.
• NCBI Official Full Name: GRB2-associated-binding protein 2 isoform b
• NCBI Official Synonym Full Names: GRB2 associated binding protein 2
• NCBI Official Symbol: GAB2
• NCBI Protein Information: GRB2-associated-binding protein 2
• UniProt Protein Name: GRB2-associated-binding protein 2
• Protein Family: GRB2-associated-binding protein
• UniProt Gene Name: GAB2
• UniProt Synonym Gene Names: KIAA0571
• UniProt Entry Name: GAB2_HUMAN

NCBI Description

This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

GAB2: an adaptor protein of the GRB2-associated binding protein family. Plays important roles in regulating the growth, differentiation, and function of many hematopoietic cell types.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: integrin-mediated signaling pathway; cell migration; phosphoinositide-mediated signaling; positive regulation of cell proliferation; innate immune response; positive regulation of mast cell degranulation; osteoclast differentiation; transmembrane receptor protein tyrosine kinase signaling pathway