Western Blot (WB)
(Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-G9a (0.25ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G9a (~160kD).)
Rabbit anti-Human, Mouse G9a Polyclonal Antibody | anti-G9a antibody
G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a,
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-G9a (0.25ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G9a (~160kD).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-G9a (0.25ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G9a (~160kD).)
Western Blot (WB)
(Western Blot Analysis (SNAP ID Technique): Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with Anti-G9a (1ug/ml). Proteins were visualized using a Snap ID, a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G9a (~160kD).)
Western Blot (WB)
(Western Blot Analysis (SNAP ID Technique): Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with Anti-G9a (1ug/ml). Proteins were visualized using a Snap ID, a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates G9a (~160kD).)
NCBI and Uniprot Product Information
NCBI Description
This gene is located in a gene cluster within the human major histocompatibility complex class III region on chromosome 6. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but recent publications show that there is only a single gene at this locus. The encoded protein is a histone methyltransferase, which may be involved in repression of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
EHMT2 iso4: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43
Chromosomal Location of Human Ortholog: 6p21.31
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleus
Molecular Function: protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)
Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA replication; histone H3-K9 methylation
Research Articles on G9a
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Product Notes
The G9a ehmt2 (Catalog #AAA6477253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a, reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's G9a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.25-2ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the G9a ehmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G9a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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