Rabbit anti-Human G6PD Polyclonal Antibody | anti-G6PD antibody

G6PD, ID (G6PD, Glucose-6-phosphate 1-dehydrogenase) (MaxLight 650)

Cat. Num. AAA6300672

• Gene Names: G6PD; G6PD1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: G6PD; Polyclonal Antibody; ID (G6PD; Glucose-6-phosphate 1-dehydrogenase) (MaxLight 650); Glucose-6-phosphate 1-dehydrogenase; anti-G6PD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-G6PD antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: G6PD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-335 amino acids from the Central region of human G6PD.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of G6PD Antibody (Center) in K562,MCF-7 cell line lysates (35ug/lane).G6PD (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(G6PD Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(G6PD Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-G6PD antibody

G6PD encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia.

Product Categories/Family for anti-G6PD antibody

Antibodies; Enzymes; Dehydrogenase

References

Louicharoen, C., et al. Science 326(5959):1546-1549(2009). Zhong, D.N., et al. Zhongguo Dang Dai Er Ke Za Zhi 11(12):970-972(2009). Tiono, A.B., et al. Am. J. Trop. Med. Hyg. 81(6):969-978(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 1203978
• NCBI GeneID: 2539
• UniProt Accession #: P11413; Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2; D3DWX9
• Molecular Weight: 59,257 Da
• NCBI Official Full Name: G6PD
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase
• NCBI Official Symbol: G6PD
• NCBI Official Synonym Symbols: G6PD1
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase, G6PD
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6PD
• UniProt Synonym Gene Names: G6PD
• UniProt Entry Name: G6PD_HUMAN

NCBI Description

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: Cell development/differentiation; Other Amino Acids Metabolism - glutathione; Carbohydrate Metabolism - pentose phosphate pathway; EC 1.1.1.49; Oxidoreductase

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centrosome; internal side of plasma membrane; membrane; intracellular membrane-bound organelle; cytoplasm; microtubule organizing center; nucleus; cytosol

Molecular Function: identical protein binding; glucose-6-phosphate dehydrogenase activity; protein binding; protein homodimerization activity; NADP binding; glucose binding

Biological Process: NADPH regeneration; response to food; pentose-phosphate shunt; erythrocyte maturation; ribose phosphate biosynthetic process; glucose 6-phosphate metabolic process; pentose-phosphate shunt, oxidative branch; glucose metabolic process; cytokine production; pathogenesis; NADP metabolic process; cholesterol biosynthetic process; response to organic cyclic substance; glutathione metabolic process; substantia nigra development; response to ethanol; regulation of neuron apoptosis; carbohydrate metabolic process; lipid metabolic process; pentose biosynthetic process

Disease: Favism, Susceptibility To; Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Malaria, Susceptibility To