Rabbit anti-Human FZR1 Polyclonal Antibody | anti-FZR1 antibody

FZR1, NT (FZR1, CDH1, FYR, FZR, KIAA1242, Fizzy-related protein homolog, CDC20-like protein 1, Cdh1/Hct1 homolog) (Azide free) (HRP)

Cat. Num. AAA6300624

• Gene Names: FZR1; FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FZR1; Polyclonal Antibody; NT (FZR1; CDH1; FYR; FZR; KIAA1242; Fizzy-related protein homolog; CDC20-like protein 1; Cdh1/Hct1 homolog) (Azide free) (HRP); Cdh1/Hct1 homolog; anti-FZR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FZR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FZR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human FZR1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FZR1 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the FZR1 antibody detected the FZR1 protein (arrow).)

Product Categories/Family for anti-FZR1 antibody

Antibodies; Cell Division Cycle (CDC)

References

Olson, J.E., et al. Breast Cancer Res. Treat. 125(1):221-228(2011) Colombo, S.L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):18868-18873(2010). Naoe, H., et al. Mol. Cell. Biol. 30(16):3994-4005(2010). Sigl, R., et al. J. Cell. Sci. 122 (PT 22), 4208-4217 (2009): Bassermann, F., et al. Cell 134(2):256-267(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 33620742
• NCBI GeneID: 51343
• NCBI Accession #: NP_057347.2
• NCBI GenBank Nucleotide #: NM_016263.3
• UniProt Accession #: Q9UM11; O75869; Q86U66; Q96NW8; Q9UI96; Q9ULH8; Q9UM10; Q9UNQ1; Q9Y2T8
• Molecular Weight: 44,874 Da
• NCBI Official Full Name: fizzy-related protein homolog isoform 2
• NCBI Official Synonym Full Names: fizzy/cell division cycle 20 related 1 (Drosophila)
• NCBI Official Symbol: FZR1
• NCBI Official Synonym Symbols: FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C
• NCBI Protein Information: fizzy-related protein homolog; CDC20-like 1b; cdh1/Hct1 homolog; CDC20-like protein 1
• UniProt Protein Name: Fizzy-related protein homolog
• Protein Family: Fizzy-related protein
• UniProt Gene Name: FZR1
• UniProt Synonym Gene Names: CDH1; FYR; FZR; KIAA1242; Fzr; hCDH1
• UniProt Entry Name: FZR_HUMAN

Uniprot Description

FZR1: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1- mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Belongs to the WD repeat CDC20/Fizzy family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; nuclear membrane; anaphase-promoting complex; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of meiosis; DNA repair; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; positive regulation of protein catabolic process; mitotic cell cycle; G2/M transition DNA damage checkpoint