Goat FZD8/frizzled 8 Polyclonal Antibody | anti-FZD8 antibody

Goat anti-FZD8/frizzled 8 Antibody

Cat. Num. AAA420018

• Gene Names: FZD8; FZ-8; hFZ8
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FZD8/frizzled 8; Polyclonal Antibody; Goat anti-FZD8/frizzled 8 Antibody; FZD8; frizzled homolog 8 (Drosophila); HGNC: 4046; FZ-8; hFZ8; frizzled (Drosophila) homolog 8; frizzled 8; FZD8 antibody; frizzled homolog 8 (Drosophila) antibody; HGNC: 4046 antibody; FZ-8 antibody; hFZ8 antibody; frizzled (Drosophila) homolog 8 antibody; frizzled 8 antibody; Frizzled 8; FZD8 / frizzled 8; anti-FZD8 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SYPKQMPLSQV
• Sequence Length: 694

• Applicable Applications for anti-FZD8 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Pancreas, shows granular cytoplasmic staining of acinar cells. Cells of the Islets of Langerhans are weakly stained, if at all. Recommended concentration, 3ug/ml.; Western Blot: Approx 75kDa band observed in human heart and human pancreas lysates (calculated MW of 73.3kDa according to NP_114072.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of human pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-)

NCBI and Uniprot Product Information

• NCBI GI #: 13994190
• NCBI GeneID: 8325
• NCBI Accession #: NP_114072.1
• NCBI GenBank Nucleotide #: NM_031866.2
• Molecular Weight: 73,300 Da
• NCBI Official Full Name: frizzled-8
• NCBI Official Synonym Full Names: frizzled class receptor 8
• NCBI Official Symbol: FZD8
• NCBI Official Synonym Symbols: FZ-8; hFZ8
• NCBI Protein Information: frizzled-8
• UniProt Protein Name: Frizzled-8
• Protein Family: Frizzled
• UniProt Gene Name: FZD8
• UniProt Synonym Gene Names: Fz-8; hFz8
• UniProt Entry Name: FZD8_HUMAN

NCBI Description

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD8: Receptor for Wnt proteins. Component of the Wnt-Fzd- LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome- sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: Golgi apparatus; integral to membrane

Molecular Function: G-protein coupled receptor activity; PDZ domain binding; protein binding; receptor binding; ubiquitin protein ligase binding; Wnt receptor activity; Wnt-protein binding

Biological Process: angiogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of JNK activity; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin