Rabbit anti-Human, Mouse FZD6 Polyclonal Antibody | anti-FZD6 antibody

FZD6 Polyclonal Antibody

Cat. Num. AAA9131002

• Gene Names: FZD6; FZ6; FZ-6; HFZ6; NDNC10
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: FZD6; Polyclonal Antibody; FZD6 Polyclonal Antibody; FZ-6; FZ6; HFZ6; NDNC10; anti-FZD6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 706

• Applicable Applications for anti-FZD6 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 - 1:2000
• Immunogen: Recombinant protein of human FZD6
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FZD6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Related Product Information for anti-FZD6 antibody

This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.

Product Categories/Family for anti-FZD6 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 215273986
• NCBI GeneID: 8323
• UniProt Accession #: O60353; Q6N0A5; Q6P9C3; Q8WXR9; B4DRN0
• Molecular Weight: 79kDa
• NCBI Official Full Name: Frizzled-6
• NCBI Official Synonym Full Names: frizzled class receptor 6
• NCBI Official Symbol: FZD6
• NCBI Official Synonym Symbols: FZ6; FZ-6; HFZ6; NDNC10
• NCBI Protein Information: frizzled-6
• UniProt Protein Name: Frizzled-6
• Protein Family: Frizzled
• UniProt Gene Name: FZD6
• UniProt Synonym Gene Names: Fz-6; hFz6

NCBI Description

This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011]

Uniprot Description

FZD6: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; ubiquitin protein ligase binding; Wnt receptor activity; Wnt-protein binding

Biological Process: negative regulation of transcription factor activity; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Nail Disorder, Nonsyndromic Congenital, 10