Rabbit anti-Human FZD3 Polyclonal Antibody | anti-FZD3 antibody

FZD3, CT (FZD3, Frizzled-3) (Biotin)

Cat. Num. AAA6300556

• Gene Names: FZD3; Fz-3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FZD3; Polyclonal Antibody; CT (FZD3; Frizzled-3) (Biotin); Frizzled-3; anti-FZD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FZD3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FZD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-525 amino acids from the C-terminal region of human FZD3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FZD3 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FZD3 antibody detected the FZD3 protein (arrow).)

Related Product Information for anti-FZD3 antibody

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development.

Product Categories/Family for anti-FZD3 antibody

Antibodies; Wnt Proteins

References

Inkster, B., et al. Neuroimage 53(3):908-917(2010). Kawano, Y., et al. Br. J. Cancer 100(7):1165-1174(2009). Gregorio, S.P., et al. Psychiatry Res 165 (1-2), 1-9 (2009): Wang, H.X., et al. Mol. Hum. Reprod. 15(1):11-17(2009). Kishimoto, M., et al. Behav Brain Funct 4, 37 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 8393378
• NCBI GeneID: 7976
• NCBI Accession #: NP_059108.1
• NCBI GenBank Nucleotide #: NM_017412.3
• UniProt Accession #: Q9NPG1
• Molecular Weight: 76,263 Da
• NCBI Official Full Name: frizzled-3
• NCBI Official Synonym Full Names: frizzled family receptor 3
• NCBI Official Symbol: FZD3
• NCBI Official Synonym Symbols: Fz-3
• NCBI Protein Information: frizzled-3; frizzled homolog 3; frizzled 3, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-3
• UniProt Gene Name: FZD3
• UniProt Synonym Gene Names: Fz-3; hFz3
• UniProt Entry Name: FZD3_HUMAN

NCBI Description

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]

Uniprot Description

FZD3: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: cell surface; cell soma; axon; cytoplasm; apical plasma membrane; dendrite; presynaptic active zone; integral to membrane; plasma membrane; lateral plasma membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; PDZ domain binding

Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; positive regulation of neuroblast proliferation; inner ear morphogenesis; hair follicle development; neural tube closure; neuron migration; establishment of planar polarity; Wnt receptor signaling pathway through beta-catenin; cell proliferation in midbrain; negative regulation of mitotic cell cycle, embryonic