Rabbit anti-Human FZD1 Polyclonal Antibody | anti-FZD1 antibody

FZD1, ID (Frizzled-1, Fz-1, hFz1, FzE1) (AP)

Cat. Num. AAA6476940

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: FZD1; Polyclonal Antibody; ID (Frizzled-1; Fz-1; hFz1; FzE1) (AP); anti-FZD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FZD1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-FZD1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 367-396 in the central region of human FZD1, UniProt Accession #NP_003496. Species sequence homology: mouse and rat
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Hela cell line lysates (35ug/lane) usingMBS629125. FZD1(arrow) was detected usingMBS629125.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue usingMBS629125, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Testing Data

()

Related Product Information for anti-FZD1 antibody

Members of the 'frizzled' family are 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif.

Product Categories/Family for anti-FZD1 antibody

Antibodies; Wnt Proteins

References

1. Quelard, D., (er) PLoS ONE 3(4), E1878 (2008). 2. Hardie, W.D., Am. J. Respir. Cell Mol. Biol. 37(3), 309-321 (2007). 3. Yang, L., J. Dermatol. Sci. 42(2), 111-119 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4503825
• NCBI GeneID: 8321
• NCBI Accession #: NP_003496
• NCBI GenBank Nucleotide #: NM_003505
• UniProt Accession #: Q9UP38; O94815; Q549T8; A4D1E8
• Molecular Weight: 71,158 Da
• NCBI Official Full Name: frizzled-1
• NCBI Official Synonym Full Names: frizzled class receptor 1
• NCBI Official Symbol: FZD1
• NCBI Protein Information: frizzled-1; Wnt receptor; frizzled 1, seven transmembrane spanning receptor; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; fz-1; fzE1; hFz1
• UniProt Protein Name: Frizzled-1
• Protein Family: Frizzled
• UniProt Gene Name: FZD1
• UniProt Synonym Gene Names: Fz-1; hFz1
• UniProt Entry Name: FZD1_HUMAN

Uniprot Description

FZD1: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Receptor, GPCR; GPCR, Fz/Smo family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: focal adhesion; cell surface; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; frizzled binding; receptor binding; PDZ domain binding

Biological Process: neuron differentiation; response to drug; G-protein coupled receptor protein signaling pathway; cell-cell signaling; epithelial cell differentiation; positive regulation of transcription, DNA-dependent; hard palate development; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway