Western Blot (WB)
(Western blot analysis of extracts of A-431 cells, using Phospho-FYN-S21 antibody at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Rabbit anti-Human FYN-S21 Polyclonal Antibody | anti-FYN antibody
Phospho-FYN-S21 Polyclonal Antibody
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of A-431 cells, using Phospho-FYN-S21 antibody at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Western Blot (WB)
(Western blot analysis of extracts of A-431 cells, using Phospho-FYN-S21 antibody at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]
Uniprot Description
Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy.
Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Oncoprotein; Kinase, protein; TK group; Src family
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: extrinsic to internal side of plasma membrane; mitochondrion; postsynaptic density; plasma membrane; actin filament; nucleus; cytosol; endosome; lipid raft
Molecular Function: tubulin binding; CD8 receptor binding; ephrin receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; peptide hormone receptor binding; G-protein-coupled receptor binding; protein-tyrosine kinase activity; T cell receptor binding; phosphoinositide 3-kinase binding; glycoprotein binding; CD4 receptor binding; ATP binding
Biological Process: axon guidance; central nervous system development; nerve growth factor receptor signaling pathway; T cell activation; viral reproduction; neuron migration; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of apoptosis; regulation of cell shape; calcium ion transport; forebrain development; ephrin receptor signaling pathway; feeding behavior; negative regulation of neuron apoptosis; cell differentiation; response to drug; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of I-kappaB kinase/NF-kappaB cascade; dendrite morphogenesis; activated T cell proliferation; learning; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; detection of mechanical stimulus involved in sensory perception of pain; response to ethanol; T cell costimulation; innate immune response; blood coagulation; negative regulation of protein catabolic process; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on FYN
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Product Notes
The FYN fyn (Catalog #AAA9128175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-FYN-S21 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FYN-S21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the FYN fyn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FYN-S21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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