Rabbit anti-Mouse, Rat Fyn Polyclonal Antibody | anti-Fyn antibody

Fyn (K207) Polyclonal Antibody

Cat. Num. AAA3003509

• Gene Names: FYN; SLK; SYN; p59-FYN
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Fyn; Polyclonal Antibody; Fyn (K207) Polyclonal Antibody; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn; FYN; anti-Fyn antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Fyn (K207) polyclonal antibody detects endogenous levels of Fyn protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 537

• Applicable Applications for anti-Fyn antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 181-230 of Human Fyn.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Fyn (K207) polyclonal antibody at 1:500 dilutionLane1:The brain tissue lysate of Mouse (30ug) Lane2:The brain tissue lysate of Rat (40ug))

Related Product Information for anti-Fyn antibody

Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.

NCBI and Uniprot Product Information

• NCBI GI #: 4503823
• NCBI GeneID: 2534
• NCBI Accession #: NP_002028.1
• NCBI GenBank Nucleotide #: NP_002028.1
• UniProt Accession #: P06241
• Molecular Weight: ~59kDa
• NCBI Official Full Name: tyrosine-protein kinase Fyn isoform a
• NCBI Official Synonym Full Names: FYN proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: FYN
• NCBI Official Synonym Symbols: SLK; SYN; p59-FYN
• NCBI Protein Information: tyrosine-protein kinase Fyn
• UniProt Protein Name: Tyrosine-protein kinase Fyn
• Protein Family: FYN-binding protein
• UniProt Gene Name: FYN
• UniProt Synonym Gene Names: SLK
• UniProt Entry Name: FYN_HUMAN

NCBI Description

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy.

Protein type: Protein kinase, TK; Oncoprotein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: extrinsic to internal side of plasma membrane; mitochondrion; postsynaptic density; plasma membrane; actin filament; cytosol; nucleus; endosome; lipid raft

Molecular Function: tubulin binding; CD8 receptor binding; ephrin receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; G-protein-coupled receptor binding; peptide hormone receptor binding; protein-tyrosine kinase activity; T cell receptor binding; phosphoinositide 3-kinase binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: axon guidance; central nervous system development; viral reproduction; T cell activation; nerve growth factor receptor signaling pathway; neuron migration; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of apoptosis; regulation of cell shape; calcium ion transport; forebrain development; ephrin receptor signaling pathway; feeding behavior; negative regulation of neuron apoptosis; cell differentiation; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; cell migration; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; dendrite morphogenesis; activated T cell proliferation; learning; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; detection of mechanical stimulus involved in sensory perception of pain; response to ethanol; T cell costimulation; innate immune response; negative regulation of protein catabolic process; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway