Western Blot (WB)
((0.3ug/ml) staining of A431 (A) and Jurkat lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FYN Polyclonal Antibody | anti-FYN antibody
Goat anti-FYN Antibody
Western Blot: Approx 55kDa band observed in lysates of cell lines A431 and Jurkat (calculated MW of 54.5kDa according to NP_694593.1). Recommended for use at 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of A431 (A) and Jurkat lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of A431 (A) and Jurkat lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]
Uniprot Description
Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy.
Protein type: Protein kinase, TK; EC 2.7.10.2; Oncoprotein; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Src family
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: actin filament; cytosol; endosome; extrinsic to internal side of plasma membrane; lipid raft; mitochondrion; nucleus; plasma membrane; postsynaptic density
Molecular Function: ATP binding; CD4 receptor binding; CD8 receptor binding; ephrin receptor binding; G-protein-coupled receptor binding; glycoprotein binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; peptide hormone receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoinositide 3-kinase binding; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; T cell receptor binding; tubulin binding
Biological Process: activated T cell proliferation; adaptive immune response; axon guidance; blood coagulation; calcium ion transport; cell differentiation; cell migration; central nervous system development; dendrite morphogenesis; detection of mechanical stimulus involved in sensory perception of pain; ephrin receptor signaling pathway; feeding behavior; forebrain development; innate immune response; learning; leukocyte migration; MAPKKK cascade; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; negative regulation of protein ubiquitination; neuron migration; peptidyl-tyrosine phosphorylation; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell proliferation; regulation of cell shape; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; response to drug; response to ethanol; stimulatory C-type lectin receptor signaling pathway; T cell activation; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction
Research Articles on FYN
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Product Notes
The FYN fyn (Catalog #AAA421211) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FYN Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FYN can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 55kDa band observed in lysates of cell lines A431 and Jurkat (calculated MW of 54.5kDa according to NP_694593.1). Recommended for use at 0.3-1ug/ml. Researchers should empirically determine the suitability of the FYN fyn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VQCKDKEATK LTE. It is sometimes possible for the material contained within the vial of "FYN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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