Rabbit FXR Polyclonal Antibody | anti-FXR antibody

Anti-FXR Antibody

Cat. Num. AAA416423

• Gene Names: NR1H4; BAR; FXR; HRR1; HRR-1; RIP14
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: FXR; Polyclonal Antibody; Anti-FXR Antibody; anti-FXR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Not yet tested in other species.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2313

• Applicable Applications for anti-FXR antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA titer: 1:1,000-1:5,000; Western blot analysis: 1:200-500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

References

(1) Papetti,M., Wood,N., Lohmar,P.D.et al. Direct Submission.Submitted (28-AUG-1996) Immunology and Hematopoiesis, Genetics Institute, 87 CambridgePark Drive, Cambridge, MA 02140, USA

NCBI and Uniprot Product Information

• NCBI GI #: 332801020
• NCBI GeneID: 9971
• NCBI Accession #: NP_005114.1
• NCBI GenBank Nucleotide #: NM_005123
• Molecular Weight: 48,795 Da
• NCBI Official Full Name: Homo sapiens nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 4
• NCBI Official Symbol: NR1H4
• NCBI Official Synonym Symbols: BAR; FXR; HRR1; HRR-1; RIP14
• NCBI Protein Information: bile acid receptor; RXR-interacting protein 14; farnesoid X nuclear receptor; farnesoid X-activated receptor; farnesol receptor HRR-1; retinoid X receptor-interacting protein 14
• UniProt Protein Name: Bile acid receptor
• UniProt Gene Name: NR1H4
• UniProt Entry Name: NR1H4_HUMAN

NCBI Description

This gene encodes a ligand-activated transcription factor, which shares structural features in common with nuclear hormone receptor family, such as a DNA-binding domain that targets the receptor to specific DNA sequences, and a ligand-binding domain, which interacts directly with the ligand and contains a ligand-dependent transcriptional activation domain. This protein functions as a receptor for bile acids, and when bound to bile acids, regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

NR1H4: Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Heterodimer of NR1H4 and RXR. After activation by agonist binding, interacts with a coactivator, NCOA1 or NCOA2. Interacts with CARM1 and SMARD1. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 12q23.1

Cellular Component: nucleoplasm

Molecular Function: ligand-dependent nuclear receptor binding; RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; zinc ion binding; bile acid binding; transcription coactivator activity; peptide binding; retinoid X receptor binding; protein binding; sequence-specific DNA binding; double-stranded DNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription corepressor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; gut development; bile acid metabolic process; response to glucose stimulus; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; response to lipopolysaccharide; gene expression; regulation of carbohydrate metabolic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction