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Western Blot (WB) (Western blotAll lanes: FXN antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:mouse brain tissueLane4:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 23kDaObserved band size: 23kDa)

Rabbit anti-Mouse, Human Fxn Polyclonal Antibody | anti-Fxn antibody

Fxn Antibody

Gene Names
Fxn; FA; X25; FARR; Frda
Reactivity
Mouse, Human
Applications
ELISA, Western Blot
Purity
>95%, Protein G Purified
Synonyms
Fxn; Polyclonal Antibody; Fxn Antibody; Frataxin; mitochondrial (Fxn) (EC 1.16.3.1) [Cleaved into: Frataxin intermediate form; Frataxin mature form]; Frda; anti-Fxn antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Form/Format
Liquid
Sequence Length
207
Applicable Applications for anti-Fxn antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Recommended dilution: WB:1:500-2000
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant Mouse Frataxin, mitochondrial protein (78-207AA)
Research Area
Tags & Cell Markers
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: FXN antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:mouse brain tissueLane4:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 23kDaObserved band size: 23kDa)

Western Blot (WB) (Western blotAll lanes: FXN antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:mouse brain tissueLane4:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 23kDaObserved band size: 23kDa)
Related Product Information for anti-Fxn antibody
Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization. Modulates the RNA-binding activity of ACO1 (By similarity).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
frataxin, mitochondrial
NCBI Official Synonym Full Names
frataxin
NCBI Official Symbol
Fxn
NCBI Official Synonym Symbols
FA; X25; FARR; Frda
NCBI Protein Information
frataxin, mitochondrial
UniProt Protein Name
Frataxin, mitochondrial
UniProt Gene Name
Fxn
UniProt Synonym Gene Names
Frda; Fxn
UniProt Entry Name
FRDA_MOUSE

Uniprot Description

FXN: Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1. Defects in FXN are the cause of Friedreich ataxia (FRDA). FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia. The disorder is usually manifest before adolescence and is generally characterized by incoordination of limb movements, dysarthria, nystagmus, diminished or absent tendon reflexes, Babinski sign, impairment of position and vibratory senses, scoliosis, pes cavus, and hammer toe. In most patients, FRDA is due to GAA triplet repeat expansions in the first intron of the frataxin gene. But in some cases the disease is due to mutations in the coding region. Belongs to the frataxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Motility/polarity/chemotaxis; EC 1.16.3.1

Cellular Component: cytoplasm; cytosol; mitochondrion

Molecular Function: 2 iron, 2 sulfur cluster binding; enzyme binding; ferric iron binding; ferrous iron binding; ferroxidase activity; iron-sulfur cluster binding; metal ion binding; oxidoreductase activity

Biological Process: adult walking behavior; aerobic respiration; cellular iron ion homeostasis; embryonic development ending in birth or egg hatching; heme biosynthetic process; ion transport; iron incorporation into metallo-sulfur cluster; iron ion homeostasis; iron-sulfur cluster assembly; mitochondrion organization and biogenesis; negative regulation of apoptosis; negative regulation of multicellular organism growth; negative regulation of organ growth; oxidative phosphorylation; positive regulation of axon extension; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of lyase activity; positive regulation of metalloenzyme activity; proprioception; protein autoprocessing; response to iron ion; transport

Research Articles on Fxn

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Product Notes

The Fxn fxn (Catalog #AAA1498536) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fxn Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fxn can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the Fxn fxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fxn, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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