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Western Blot (WB) (FX4L1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the FX4L1 antibody detected the FX4L1 protein (arrow).)

Rabbit anti-Human FX4L1 Polyclonal Antibody | anti-FX4L1 antibody

FX4L1, NT (FOXD4L1, Forkhead box protein D4-like 1) (Azide free) (HRP)

Gene Names
FOXD4L1; FOXD5; bA395L14.1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
FX4L1; Polyclonal Antibody; NT (FOXD4L1; Forkhead box protein D4-like 1) (Azide free) (HRP); FOXD4L1; Forkhead box protein D4-like 1; anti-FX4L1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-FX4L1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
FX4L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FX4L1.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FX4L1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the FX4L1 antibody detected the FX4L1 protein (arrow).)

Western Blot (WB) (FX4L1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the FX4L1 antibody detected the FX4L1 protein (arrow).)

Flow Cytometry (FC/FACS)

(FX4L1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (FX4L1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-FX4L1 antibody
This gene is a member of the forkhead/winged-helix (FOX) family of transcription factors with highly conserved FOX DNA-binding domains. Members of the FOX family of transcription factors are regulators of embryogenesis and may play a role in human cancer. This gene lies in a region of chromosome 2 that surrounds the site where two ancestral chromosomes fused to form human chromosome 2. This region is duplicated elsewhere in the human genome, primarily in subtelomeric and pericentromeric locations, thus mutiple copies of this gene have been found.
Product Categories/Family for anti-FX4L1 antibody
References
Hillier, L.W., et al. Nature 434(7034):724-731(2005). Wong, A., et al. Genomics 84(2):239-247(2004). Fan, Y., et al. Genome Res. 12(11):1663-1672(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,610 Da
NCBI Official Full Name
forkhead box protein D4-like 1
NCBI Official Synonym Full Names
forkhead box D4-like 1
NCBI Official Symbol
FOXD4L1
NCBI Official Synonym Symbols
FOXD5; bA395L14.1
NCBI Protein Information
forkhead box protein D4-like 1; FOXD4-like 1; forkhead box D4 like 1
UniProt Protein Name
Forkhead box protein D4-like 1
UniProt Gene Name
FOXD4L1
UniProt Synonym Gene Names
FOXD4-like 1
UniProt Entry Name
FX4L1_HUMAN

NCBI Description

This gene is a member of the forkhead/winged-helix (FOX) family of transcription factors with highly conserved FOX DNA-binding domains. Members of the FOX family of transcription factors are regulators of embryogenesis and may play a role in human cancer. This gene lies in a region of chromosome 2 that surrounds the site where two ancestral chromosomes fused to form human chromosome 2. This region is duplicated elsewhere in the human genome, primarily in subtelomeric and pericentromeric locations, thus mutiple copies of this gene have been found. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXD4L1:

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

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Product Notes

The FX4L1 foxd4l1 (Catalog #AAA6300448) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FX4L1, NT (FOXD4L1, Forkhead box protein D4-like 1) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FX4L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FX4L1 foxd4l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FX4L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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