Goat anti-Human FURIN Polyclonal Antibody

GOAT ANTI HUMAN FURIN (C-TERMINAL)

Cat. Num. AAA225873

• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: FURIN; Polyclonal Antibody; GOAT ANTI HUMAN FURIN (C-TERMINAL); Furin antibody; anti-FURIN antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FURIN
• Form/Format: Purified IgG, liquid. TRIS buffered saline; Antiserum to human Furin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Concentration: IgG concentration 0.5mg/ml (varies by lot)

• Applicable Applications for anti-FURIN antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1/128000; WB: 0.01-0.03ug/ml; Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.; Western Blotting: This product detects a band of approximately 110kDa in HEK293 lysate transiently expressing Human Furin tagged with myc and DYKDDDDK.
• Species Cross-Reactivity: Mouse, Rat, Dog, Pig, Bovine
• Immunogen: Synthetic peptide sequence C-EDEGRGERTAFIKDQ, from within the C-Terminal region of Furin (NP_002560.1).
• Preservative Stabilizers: 0.02% Sodium Azide (NaN3); Preservative Stabilizers
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; Storage in frost-free freezers is not recommended.; This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Related Product Information for anti-FURIN antibody

Goat anti Human furin antibody recognizes an epitope within the C-Terminal (CT) region of furin, otherwise known as PACE (Paired basic Amino acid Cleaving Enzyme). Furin is a calcium-dependent serine endoprotease and proprotein convertase, which plays a crucial role in the processing of latent precursor proteins into their biologically active forms, such as during the Notch signaling pathway.
The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.
Furin is a critical enzyme during the S1 cleavage of pre-Notch proteins within the golgi apparatus, to convert the nascent form into the mature heterodimeric Notch receptor and the non-covalently associated intracellular domain. These are then transported to the cell surface, in preparation for ligand-receptor binding and further S2 and S3 cleavage, resulting in the release and translocation of the intracellular domain to the nucleus.

References

1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.Nat Rev Mol Cell Biol. 7 (9): 678-89.