Rabbit anti-Human Fucosyltransferase 1 Polyclonal Antibody | anti-FUT1 antibody

Fucosyltransferase 1 (FUT1, 2-alpha-L-fucosyltransferase 1, Alpha(1,2)FT 1, Blood Group H alpha 2-fucosyltransferase, Galactoside 2-alpha-L-fucosyltransferase 1, GDP-L-fucose:beta-D-galactoside, H, HH, HSC) (Biotin)

Cat. Num. AAA6477174

• Gene Names: FUT1; H; HH; HSC
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Fucosyltransferase 1; Polyclonal Antibody; Fucosyltransferase 1 (FUT1; 2-alpha-L-fucosyltransferase 1; Alpha(1; 2)FT 1; Blood Group H alpha 2-fucosyltransferase; Galactoside 2-alpha-L-fucosyltransferase 1; GDP-L-fucose:beta-D-galactoside; H; HH; HSC) (Biotin); Fucosyltransferase 1 (Galactoside 2-alpha-L-fucosyltransferase; H Blood Group); EC=2.4.1.69; anti-FUT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FUT1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FUT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human FUT1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FUT1 antibody (Center) in CEM cell line lysates (35ug/lane). FUT1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(FUT1 Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FUT1 antibody

Antibodies; Enzymes; Glycosyltransferase

References

1. Moehler, T.M., J. Cell. Physiol. 215(1), 27-36 (2008). 2. Moore, G.T., Mol. Immunol. 45(8), 2401-2410 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4503805
• NCBI GeneID: 2523
• NCBI Accession #: NP_000139
• NCBI GenBank Nucleotide #: NM_000148
• UniProt Accession #: P19526
• Molecular Weight: 41
• NCBI Official Full Name: galactoside 2-alpha-L-fucosyltransferase 1
• NCBI Official Synonym Full Names: fucosyltransferase 1 (H blood group)
• NCBI Official Symbol: FUT1
• NCBI Official Synonym Symbols: H; HH; HSC
• NCBI Protein Information: galactoside 2-alpha-L-fucosyltransferase 1
• UniProt Protein Name: Galactoside 2-alpha-L-fucosyltransferase 1
• Protein Family: Galactoside 2-alpha-L-fucosyltransferase
• UniProt Gene Name: FUT1
• UniProt Synonym Gene Names: H; HSC
• UniProt Entry Name: FUT1_HUMAN

NCBI Description

This gene encodes a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the synthesis of soluble A and B antigens. This is one of two genes encoding the galactoside 2-L-fucosyltransferase enzyme. Mutations in this gene are a cause of the H-Bombay blood group. [provided by RefSeq, Aug 2016]

Uniprot Description

FUT1: Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. Belongs to the glycosyltransferase 11 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; Transferase; EC 2.4.1.69; Glycan Metabolism - glycosphingolipid biosynthesis - globo series

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: Golgi apparatus; integral to plasma membrane; membrane

Molecular Function: fucosyltransferase activity; galactoside 2-alpha-L-fucosyltransferase activity

Biological Process: carbohydrate metabolic process; L-fucose catabolic process; protein amino acid glycosylation