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Rabbit FTSH2 Polyclonal Antibody | anti-FTSH2 antibody

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FTSH2 Polyclonal Antibody

Gene Names
VAR2; F7F1.16; F7F1_16; FTSH2; VAR2; VARIEGATED 2
Reactivity
Arabidopsis thaliana (Mouse-ear cress)
Applications
ELISA, Western Blot
Purity
Protein A/G
Synonyms
FTSH2; Polyclonal Antibody; Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FTSH2 Polyclonal Antibody; FTSH2 Antibody; ATP-dependent zinc metalloprotease FTSH 2; chloroplastic; AtFTSH2; EC 3.4.24.-; Protein VARIEGATED 2; FTSH2 VAR2 At2g30950 F7F1.16; anti-FTSH2 antibody
Ordering
Host
Rabbit
Reactivity
Arabidopsis thaliana (Mouse-ear cress)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A/G
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-FTSH2 antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
Immunogen
Recombinant Arabidopsis thaliana FTSH2 protein
Conjugation
Non-conjugate
Modification
Unmodified
Deliverables
1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
Quality Guarantee
1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
Complementary Antibody
Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,157 Da
NCBI Official Full Name
cell division protease ftsH-2
NCBI Official Symbol
VAR2
NCBI Official Synonym Symbols
F7F1.16; F7F1_16; FTSH2; VAR2; VARIEGATED 2
NCBI Protein Information
cell division protease ftsH-2
UniProt Protein Name
ATP-dependent zinc metalloprotease FTSH 2, chloroplastic
UniProt Gene Name
FTSH2
UniProt Synonym Gene Names
VAR2; AtFTSH2
UniProt Entry Name
FTSH2_ARATH

NCBI Description

Metalloprotease that functions in thylakoid membrane biogenesis. Involved in the repair of PSII following damaged incurred during photoinhibition. Forms a complex with VAR1. Mutants show a variegated phenotype, which decreases during development. Transcript and protein levels increase with light intensity.

Uniprot Description

Function: Part of a complex that function as an ATP-dependent zinc metallopeptidase. Involved in the thylakoid formation and in the removal of damaged D1 in the photosystem II, preventing cell death under high-intensity light conditions, but not involved in thermotolerance. Ref.1 Ref.7 Ref.8 Ref.13

Cofactor: Binds 1 zinc ion per subunit

By similarity.

Subunit structure: Interacts with CHIP and FTSH5. Heterohexamers with FTSH1, FTSH5 and FTSH8. May also form homooligomers. Ref.8 Ref.9 Ref.12 Ref.14

Subcellular location: Plastid › chloroplast thylakoid membrane; Single-pass membrane protein; Stromal side Ref.8.

Tissue specificity: Expressed in cotyledons, cauline and rosette leaves, stems, sepals, flovers and siliques. Very low in roots. Ref.9

Developmental stage: Low expression in cotyledons, increasing with leaves development. Ref.11

Induction: By cold and high light. Ref.10

Domain: The conserved lumenal (CL) domain (83-161) is present only in some FtsH homologs from organisms performing oxygenic photosynthesis. Ref.7

Post-translational modification: The FTSH2 precursor is ubiquitinated by CHIP in the cytoplasm.

Disruption phenotype: Leaf-variegated. Mutations can be complemented by overexpression of FTSH8. The presence of both FTSH1 or FTSH5 (subunit type A) and FTSH2 or FTSH8 (subunit type B) is essential for an active complex formation. Ref.1

Sequence similarities: In the N-terminal section; belongs to the AAA ATPase family.In the C-terminal section; belongs to the peptidase M41 family.

Sequence caution: The sequence AAK73957.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on FTSH2

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Product Notes

The FTSH2 ftsh2 (Catalog #AAA7182899) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) FTSH2 Polyclonal Antibody reacts with Arabidopsis thaliana (Mouse-ear cress) and may cross-react with other species as described in the data sheet. AAA Biotech's FTSH2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the FTSH2 ftsh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FTSH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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