Western Blot (WB)
(FTO antibody (pAb) tested by Western blot. Detection of FTO by Western blot analysis (30 ug per lane). Lane 1: Nuclear extract of Caco-2 cells. Lane 2: Nuclear extract of HEK-293 cells. Both probed with FTO antibody (pAb) at a 1:500 dilution.)
Rabbit anti-Human FTO Polyclonal Antibody | anti-FTO antibody
FTO antibody (pAb)
Gene Names
FTO; MGC5149; KIAA1752
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
FTO; Polyclonal Antibody; FTO antibody (pAb); ALKBH9; anti-FTO antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-FTO antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
WB: 1:500-1:2,000 dilution
Immunogen
This antibody was raised against a peptide within the N-terminal region of human FTO.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(FTO antibody (pAb) tested by Western blot. Detection of FTO by Western blot analysis (30 ug per lane). Lane 1: Nuclear extract of Caco-2 cells. Lane 2: Nuclear extract of HEK-293 cells. Both probed with FTO antibody (pAb) at a 1:500 dilution.)
Western Blot (WB)
(FTO antibody (pAb) tested by Western blot. Detection of FTO by Western blot analysis (30 ug per lane). Lane 1: Nuclear extract of Caco-2 cells. Lane 2: Nuclear extract of HEK-293 cells. Both probed with FTO antibody (pAb) at a 1:500 dilution.)
Related Product Information for anti-FTO antibody
Short Description: Our FTO antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: FTO (fat mass and obesity associated) is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. This protein has high activity towards single-stranded RNA containing 3-methyluracil and single-stranded DNA containing 3-methylthymine. FTO has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and has no activity towards 1-methylguanine or double-stranded DNA. FTO contributes to the regulation of the global metabolic rate, energy expenditure, energy homeostasis, regulation of body size and body fat accumulation. FTO has recently been shown to have the highest activity towards demethylation of 6-methyladeninine in single-stranded RNA suggesting that FTO is involved in processing of nuclear RNAs and may play an important role in RNA epigenetics.
Background: FTO (fat mass and obesity associated) is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. This protein has high activity towards single-stranded RNA containing 3-methyluracil and single-stranded DNA containing 3-methylthymine. FTO has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and has no activity towards 1-methylguanine or double-stranded DNA. FTO contributes to the regulation of the global metabolic rate, energy expenditure, energy homeostasis, regulation of body size and body fat accumulation. FTO has recently been shown to have the highest activity towards demethylation of 6-methyladeninine in single-stranded RNA suggesting that FTO is involved in processing of nuclear RNAs and may play an important role in RNA epigenetics.
Product Categories/Family for anti-FTO antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,282 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase FTO
NCBI Official Synonym Full Names
fat mass and obesity associated
NCBI Official Symbol
FTO
NCBI Official Synonym Symbols
MGC5149; KIAA1752
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; OTTHUMP00000213475; fat mass and obesity-associated protein
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase FTO
Protein Family
UniProt Gene Name
FTO
UniProt Synonym Gene Names
KIAA1752
UniProt Entry Name
FTO_HUMAN
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Product Notes
The FTO fto (Catalog #AAA388893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FTO antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FTO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution. Researchers should empirically determine the suitability of the FTO fto for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FTO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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