Rabbit FREAC3 Polyclonal Antibody | anti-FREAC3 antibody

FREAC3 Polyclonal Antibody

Cat. Num. AAA3015493

• Gene Names: FOXC1; ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• Reactivity: Human, Mouse, Rat, Chicken, Dog, Cow, Horse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: affinity purified by Protein A
• Synonyms: FREAC3; Polyclonal Antibody; FREAC3 Polyclonal Antibody; ARA; FKH L7; FKHL 7; FKHL7; Forkhead (Drosophila) like 7; Forkhead; forkhead box C1; Forkhead box protein C1; Forkhead drosophila homolog like 7; Forkhead like 7; Forkhead related activator 3; Forkhead related protein FKHL7; Forkhead related transcription factor 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FOX C1; FOXC 1; FOXC1; FOXC1_HUMAN; FREAC 3; FREAC-3; homolog-like 7; IGDA; IHG 1; IHG1; IRID 1; IRID1; Iridogoniodysgenesis type 1; Myeloid factor delta; anti-FREAC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Chicken, Dog, Cow, Horse
• Clonality: Polyclonal
• Specificity: FREAC3 Polyclonal Antibody detects endogenous levels of FREAC3 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-FREAC3 antibody: Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM)
• Application Notes: IHC-P: 1:100-500; IHC-F: 1:100-500; FC/FACS/FCM: 1ug/Test
• Immunogen: KLH conjugated synthetic peptide derived from human FOXC1/FREAC3:101-200/553
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation: Anti-FOXC1/FREAC3 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C)

Related Product Information for anti-FREAC3 antibody

Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees.Involvement in disease; Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3); also known as Axenfeld-Rieger syndrome (ARS) or Axenfeld syndrome or Axenfeld anomaly. It is characterized by posterior corneal embryotoxon, prominent Schwalbe line and iris adhesion to the Schwalbe line. Other features may be hypertelorism (wide spacing of the eyes), hypoplasia of the malar bones, congenital absence of some teeth and mental retardation. When associated with tooth anomalies, the disorder is known as Rieger syndrome. Glaucoma is a progressive blinding condition that occurs in approximately half of patients with Axenfeld-Rieger malformations.

NCBI and Uniprot Product Information

• NCBI GI #: 119395716
• NCBI GeneID: 2296
• NCBI Accession #: NP_001444.2
• NCBI GenBank Nucleotide #: NM_001453.2
• UniProt Accession #: Q12948; Q86UP7; Q9BYM1; Q9NUE5; Q9UDD0; Q9UP06
• Molecular Weight: 56,789 Da
• NCBI Official Full Name: forkhead box protein C1
• NCBI Official Synonym Full Names: forkhead box C1
• NCBI Official Symbol: FOXC1
• NCBI Official Synonym Symbols: ARA; IGDA; IHG1; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
• NCBI Protein Information: forkhead box protein C1; myeloid factor-delta; forkhead-related activator 3; forkhead-related protein FKHL7; forkhead, drosophila, homolog-like 7; forkhead-related transcription factor 3; forkhead/winged helix-like transcription factor 7
• UniProt Protein Name: Forkhead box protein C1
• UniProt Gene Name: FOXC1
• UniProt Synonym Gene Names: FKHL7; FREAC3; FREAC-3
• UniProt Entry Name: FOXC1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXC1: Binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Monomer. Expressed in all tissues and cell lines examined.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleoplasm; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; DNA bending activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; collagen fibril organization; neural crest cell development; glycosaminoglycan metabolic process; paraxial mesoderm formation; positive regulation of transcription, DNA-dependent; heart development; cardiac muscle cell proliferation; ovarian follicle development; regulation of transcription, DNA-dependent; ureteric bud development; germ cell migration; ventricular cardiac muscle morphogenesis; negative regulation of mitotic cell cycle; lacrimal gland development; regulation of blood vessel size; skeletal development; mesenchymal cell development; ossification; somitogenesis; Notch signaling pathway; cell migration; camera-type eye development; in utero embryonic development; regulation of organ growth; odontogenesis of dentine-containing teeth; cell proliferation; eye development; artery morphogenesis; embryonic heart tube development; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; brain development; vascular endothelial growth factor receptor signaling pathway; lymph vessel development

Disease: Axenfeld-rieger Syndrome, Type 3; Iridogoniodysgenesis, Type 1