Western Blot (WB)
((1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FOXO4/MLLT7 Polyclonal Antibody | anti-FOXO4 antibody
Goat anti-FOXO4/MLLT7 Antibody
Western Blot: Approx 60-65kDa band observed in lysates of cell line K562 (calculated MW of 53.7kDa according to NP_005929.2). Recommended concentration: 1-3ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant FOXO4 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml.
Western Blot (WB)
((1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Testing Data
((0.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml).)
Testing Data
((0.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
Uniprot Description
FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.
Protein type: Transcription factor; Oncoprotein; DNA-binding
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function: beta-catenin binding; DNA binding; enzyme binding; identical protein binding; protein binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: cell cycle arrest; insulin receptor signaling pathway; mitotic cell cycle G2/M transition DNA damage checkpoint; muscle development; negative regulation of angiogenesis; negative regulation of cell proliferation; negative regulation of smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; stem cell differentiation; transcription from RNA polymerase II promoter
Research Articles on FOXO4
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Product Notes
The FOXO4 foxo4 (Catalog #AAA420582) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXO4/MLLT7 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXO4/MLLT7 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 60-65kDa band observed in lysates of cell line K562 (calculated MW of 53.7kDa according to NP_005929.2). Recommended concentration: 1-3ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant FOXO4 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the FOXO4 foxo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EAASQDRMPQ DLD. It is sometimes possible for the material contained within the vial of "FOXO4/MLLT7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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