Western Blot (WB)
(Western Blot Analysis: Representative lot data. Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-FOXO3A (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein FOXO3A (~migrate at 80kD).)
Rabbit anti-Human FOXO3A Polyclonal Antibody | anti-FOXO3A antibody
FOXO3A (Forkhead box O3, AF6q21, AF6q21 protein, DKFZp781A0677, FKHRL1, FKHRL1P2, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1, FOXO2, MGC12739, MGC31925) (MaxLight 650)
WB: 1:500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot Analysis: Representative lot data. Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-FOXO3A (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein FOXO3A (~migrate at 80kD).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane, and probed with anti-FOXO3A (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein FOXO3A (~migrate at 80kD).)
Immunocytochemistry (ICC)
(Immunocytochemistry: Representative lot data. Confocal fluorescent analysis of HeLa cells using anti-FOXO3A antibody (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nuclei are stained with DAPI (Blue). Antibody shows positive nuclear staining.)
Immunocytochemistry (ICC)
(Immunocytochemistry: Representative lot data. Confocal fluorescent analysis of HeLa cells using anti-FOXO3A antibody (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nuclei are stained with DAPI (Blue). Antibody shows positive nuclear staining.)
NCBI and Uniprot Product Information
Uniprot Description
FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.
Protein type: Oncoprotein; Autophagy; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity; protein kinase binding
Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; initiation of primordial ovarian follicle growth; antral ovarian follicle growth; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; ovulation from ovarian follicle; glucose homeostasis; oocyte maturation; regulation of translation; regulation of transcription from RNA polymerase II promoter; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter
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Product Notes
The FOXO3A foxo3 (Catalog #AAA6477081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FOXO3A (Forkhead box O3, AF6q21, AF6q21 protein, DKFZp781A0677, FKHRL1, FKHRL1P2, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1, FOXO2, MGC12739, MGC31925) (MaxLight 650) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FOXO3A can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). ICC: 1:500 WB: 1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FOXO3A foxo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXO3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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