Rabbit FOXO3 Polyclonal Antibody | anti-FOXO3 antibody

FOXO3 Antibody

Cat. Num. AAA151452

• Gene Names: FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: FOXO3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: FOXO3; Polyclonal Antibody; FOXO3 Antibody; FOXO3 Antibody: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Forkhead box protein O3; AF6q21 protein; anti-FOXO3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: FOXO3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; FOXO3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 673

• Applicable Applications for anti-FOXO3 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 ?g/mL. Antibody can also be used for immunocytochemistry starting at 4 ?g/mL. Immunofluorescence start at 20 ?g/mL. Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: FOXO3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human FOXO3.; The immunogen is located within amino acids 40 - 90 of FOXO3.
• Positive Control: 1) Cat. No. MBS151677 - A20 Cell Lysate; 2) Cat. No. MBS154228 - A-20 Cell Slide
• User Note: Optimal dilutions for each application to be determined by the researcher.
• Preparation and Storage: FOXO3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(FOXO3 Antibody)

Immunocytochemistry (IHC)

(Immunocytochemistry of FOXO3 in A-20 cells with FOXO3 antibody at 4 μg/mL.)

Related Product Information for anti-FOXO3 antibody

FOXO3 Antibody: FOXO3 is a ubiquitously expressed 75 kDa protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. FOXO3 is involved with mTOR in the regulation of autophagy in skeletal muscle, and activates protein degradation in atrophying muscle cells. FOXO3 has also been implicated in several neurodegenerative disorders including aging, neuromuscular disease, systemic lupus erythmatosus, stroke and diabetic complications.

NCBI and Uniprot Product Information

• NCBI GI #: 42519916
• NCBI GeneID: 2309
• NCBI Accession #: NP_963853
• NCBI GenBank Nucleotide #: NM_201559.2
• UniProt Accession #: O43524; O15171; Q5T2I7; Q9BZ04; B4DVZ6; E1P5E6
• NCBI Official Full Name: forkhead box protein O3
• NCBI Official Synonym Full Names: forkhead box O3
• NCBI Official Symbol: FOXO3
• NCBI Official Synonym Symbols: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• NCBI Protein Information: forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• UniProt Protein Name: Forkhead box protein O3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO3
• UniProt Synonym Gene Names: FKHRL1; FOXO3A
• UniProt Entry Name: FOXO3_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: DNA-binding; Autophagy; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; protein kinase binding; transcription factor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; nerve growth factor receptor signaling pathway; initiation of primordial ovarian follicle growth; antral ovarian follicle growth; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; ovulation from ovarian follicle; oocyte maturation; regulation of transcription from RNA polymerase II promoter; regulation of translation; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter