Rabbit anti-Human FOXN4 Polyclonal Antibody | anti-FOXN4 antibody

FOXN4, NT (FOXN4, Forkhead box protein N4) (Azide free) (HRP)

Cat. Num. AAA6299931

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FOXN4; Polyclonal Antibody; NT (FOXN4; Forkhead box protein N4) (Azide free) (HRP); Forkhead box protein N4; anti-FOXN4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FOXN4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FOXN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human FOXN4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FOXN4 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the FOXN4 antibody detected the FOXN4 protein (arrow).)

Related Product Information for anti-FOXN4 antibody

The specific function of the protein remains unknown.

Product Categories/Family for anti-FOXN4 antibody

Antibodies; Transcription Factors

References

Hirschfield, G.M., et al. N. Engl. J. Med. 360(24):2544-2555(2009). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Colicelli, J. Sci. STKE 2004 (250), RE13 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 166795280
• NCBI GeneID: 121643
• NCBI Accession #: NP_998761.2
• NCBI GenBank Nucleotide #: NM_213596.2
• UniProt Accession #: Q96NZ1; Q6ZMR4; Q96NZ0
• Molecular Weight: 55,215 Da
• NCBI Official Full Name: forkhead box protein N4
• NCBI Official Synonym Full Names: forkhead box N4
• NCBI Official Symbol: FOXN4
• NCBI Protein Information: forkhead box protein N4; forkhead/winged helix transcription factor FOXN4
• UniProt Protein Name: Forkhead box protein N4
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXN4
• UniProt Entry Name: FOXN4_HUMAN

NCBI Description

Members of the winged-helix/forkhead family of transcription factors, such as FOXN4, are characterized by a 110-amino acid DNA-binding domain that can fold into a variant of the helix-turn-helix motif consisting of 3 alpha helices flanked by 2 large loops or wings. These transcription factors are involved in a variety of biologic processes as key regulators in development and metabolism (Li et al., 2004 [PubMed 15363391]).[supplied by OMIM, Mar 2008]

Uniprot Description

FOXN4: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: nucleus

Molecular Function: chromatin binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; heart looping; regulation of heart contraction