Western Blot (WB)
(MBS420788 (2ug/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35ug protein in RIPA buffer). Detected by chemiluminescence.)
Goat FOXL2/BPES Polyclonal Antibody | anti-FOXL2 antibody
Goat anti-FOXL2/BPES Antibody
Western Blot: Approx 35kDa band observed in lysates of cell lines K562 and HeLa, whichwas successfully blocked by incubation with the immunizing peptide (calculated MW of38.8kDa according to Human NP_075555.1). Recommended concentration 1-2ug/ml.Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
Western Blot (WB)
(MBS420788 (2ug/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35ug protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS420788 (2ug/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35ug protein in RIPA buffer). Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(MBS420788 (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citratebuffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS420788 (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citratebuffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jun 2009]
Uniprot Description
FOXL2: Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans- differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Is a regulator of CYP19 expression. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element. Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES); also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II. Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3). An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 3q23
Cellular Component: nucleus
Molecular Function: caspase regulator activity; DNA binding; estrogen receptor binding; protein binding; transcription factor activity; ubiquitin conjugating enzyme binding
Biological Process: cell differentiation; DNA fragmentation during apoptosis; embryonic eye morphogenesis; extraocular skeletal muscle development; female somatic sex determination; granulosa cell differentiation; menstruation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; oocyte growth; ovarian follicle development; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of follicle-stimulating hormone secretion; positive regulation of luteinizing hormone secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; single fertilization; transcription from RNA polymerase II promoter; uterus development
Disease: Blepharophimosis, Ptosis, And Epicanthus Inversus; Premature Ovarian Failure 3
Research Articles on FOXL2
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Product Notes
The FOXL2 foxl2 (Catalog #AAA420788) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FOXL2/BPES Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FOXL2/BPES can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 35kDa band observed in lysates of cell lines K562 and HeLa, whichwas successfully blocked by incubation with the immunizing peptide (calculated MW of38.8kDa according to Human NP_075555.1). Recommended concentration 1-2ug/ml.Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml. Researchers should empirically determine the suitability of the FOXL2 foxl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DSKTGALHSR LDL. It is sometimes possible for the material contained within the vial of "FOXL2/BPES, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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