Rabbit FoxK1 Polyclonal Antibody | anti-FoxK1 antibody

FoxK1 (G698) Polyclonal Antibody

Cat. Num. AAA3002424

• Gene Names: FOXK1; FOXK1L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FoxK1; Polyclonal Antibody; FoxK1 (G698) Polyclonal Antibody; FHX; Fork head; Forkhead box protein K1; FOXJ2 forkhead factor; FOXK1; MNF; Myocyte nuclear factor; anti-FoxK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FoxK1 (G698) polyclonal antibody detects endogenous levels of FoxK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 733

• Applicable Applications for anti-FoxK1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 668-720 of Human FoxK1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FoxK1 (G698) pAb at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug) Lane6:C6 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of FoxK1 (G698) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-FoxK1 antibody

FOXK1 is expressed in these cells and regulates cell cycle progression through an interaction with its downstream target the cyclin-dependent kinase inhibitor p21 (CIP). Loss of FOXK1 in mice results in growth retardation and a severe impairment in skeletal muscle regeneration following injury. FOXK1 also shows expression in immature tissues of brain, eye, heart, lung and thymus. It also is predominantly expressed in many malignant tissues, such as tumors of the brain, colon and lymph node.

NCBI and Uniprot Product Information

• NCBI GI #: 82546824
• NCBI GeneID: 221937
• NCBI Accession #: NP_001032242.1
• NCBI GenBank Nucleotide #: NP_001032242.1
• UniProt Accession #: P85037
• Molecular Weight: ~ 75, 97kDa
• NCBI Official Full Name: forkhead box protein K1
• NCBI Official Synonym Full Names: forkhead box K1
• NCBI Official Symbol: FOXK1
• NCBI Official Synonym Symbols: FOXK1L
• NCBI Protein Information: forkhead box protein K1
• UniProt Protein Name: Forkhead box protein K1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXK1
• UniProt Synonym Gene Names: MNF; MNF
• UniProt Entry Name: FOXK1_HUMAN

Uniprot Description

FOXK1: Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; muscle development; positive regulation of transcription, DNA-dependent; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; cell differentiation