Goat anti-Bovine Folate Binding Protein Polyclonal Antibody | anti-FOLR1 antibody

Folate Binding Protein (HRP)

Cat. Num. AAA627022

• Gene Names: FOLR1; FBP; FOLR
• Reactivity: Bovine
• Applications: ELISA, Western Blot, Immunohistochemistry, Gel Super Shift Assay
• Purity: Highly Purified; Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
• Synonyms: Folate Binding Protein; Polyclonal Antibody; Folate Binding Protein (HRP); Anti -Folate Binding Protein (HRP); anti-FOLR1 antibody

• Host: Goat
• Reactivity: Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum as well as puri ed and partially puri ed Folate Binding Protein [bovine milk]. Crossreactivity against Folate Binding Protein from other sources is unknown.
• Purity/Purification: Highly Purified; Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 10mg/ml polyethylene glycol (PEG-8000), and 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FOLR1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Immunoelectrophoresis, Western Blot, Dot Blot, ELISA, Immunomicroscopy and Immunohistochemistry.; Dilution: ELISA: 1:2000 to 1:12,000 Assay against 1ug Folate Binding Protein (Bovine Milk) using ATBS as a substrate for 30 minutes at RT.
• Immunogen: Folate Binding Protein (Bovine Milk).
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Product Categories/Family for anti-FOLR1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 9257213
• NCBI GeneID: 2348
• NCBI Accession #: NP_057941.1
• NCBI GenBank Nucleotide #: NM_016729.2
• UniProt Accession #: P15328; Q53EW2; Q6FGT8; Q6LC90; Q9UCT2
• Molecular Weight: 29,819 Da
• NCBI Official Full Name: folate receptor alpha
• NCBI Official Synonym Full Names: folate receptor 1 (adult)
• NCBI Official Symbol: FOLR1
• NCBI Official Synonym Symbols: FBP; FOLR
• NCBI Protein Information: folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18
• UniProt Protein Name: Folate receptor alpha
• Protein Family: Folate receptor
• UniProt Gene Name: FOLR1
• UniProt Synonym Gene Names: FOLR; FR-alpha; FBP
• UniProt Entry Name: FOLR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

FOLR1: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain- specific folate deficiency early in life. Onset is apparent in late infancy with severe developmental regression, movement disturbances, epilepsy, and leukodystrophy. Recognition and diagnosis of this disorder is critical because folinic acid therapy can reverse the clinical symptoms and improve brain abnormalities and function. Belongs to the folate receptor family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q13.3-q14.1

Cellular Component: anchored to external side of plasma membrane; cell surface; clathrin-coated vesicle; membrane; brush border membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; endosome

Molecular Function: folic acid transporter activity; receptor activity; folic acid binding

Biological Process: receptor-mediated endocytosis; folic acid metabolic process; folic acid transport

Disease: Neurodegeneration Due To Cerebral Folate Transport Deficiency