Goat FNBP1 / FBP17 Polyclonal Antibody | anti-FNBP1 antibody

Goat Polyclonal to Human FNBP1 / FBP17

Cat. Num. AAA244833

• Gene Names: FNBP1; FBP17
• Reactivity: Bat, Gibbon, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat, Zebrafish; Predicted Reactivity: Chicken, Xenopus (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FNBP1 / FBP17; Polyclonal Antibody; Goat Polyclonal to Human FNBP1 / FBP17; Anti-FNBP1 / FBP17 Antibody (Internal) IHC-plus; FNBP1; FBP17; HFBP17; KIAA0554; Formin-binding protein 1; Formin-binding protein 17; Formin binding protein 1; Human FNBP1; anti-FNBP1 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat, Zebrafish; Predicted Reactivity: Chicken, Xenopus (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human FNBP1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 617

• Applicable Applications for anti-FNBP1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml), WB (1:32000, 0.25 - 2 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide KQLESSKRRFERDC from an internal region of human FNBP1 (NP_055848.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Zebrafish (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: FNBP1 / FBP17 antibody was raised against synthetic peptide KQLESSKRRFERDC from an internal region of human FNBP1 (NP_055848.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Zebrafish (100%); Turkey, Chicken, Platypus, Xenopus, Pufferfish (93%); Gorilla, Orangutan (86%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FNBP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-FNBP1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody staining (0.25 ug/ml) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 38524622
• NCBI GeneID: 23048
• NCBI Accession #: NP_055848.1
• NCBI GenBank Nucleotide #: NM_015033.2
• UniProt Accession #: Q96RU3; O60301; Q3MIN8; Q5TC87; Q5TC88; Q6P658; Q7LGG2; Q9H8H8; Q9NWD1; B7ZL12; E9PGQ4
• Molecular Weight: 70,752 Da
• NCBI Official Full Name: formin-binding protein 1
• NCBI Official Synonym Full Names: formin binding protein 1
• NCBI Official Symbol: FNBP1
• NCBI Official Synonym Symbols: FBP17
• NCBI Protein Information: formin-binding protein 1; formin-binding protein 17
• UniProt Protein Name: Formin-binding protein 1
• Protein Family: Formin-binding protein
• UniProt Gene Name: FNBP1
• UniProt Synonym Gene Names: FBP17; KIAA0554; hFBP17
• UniProt Entry Name: FNBP1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain, a proline-rich motif, a second coiled-coil domain, a Rho family protein-binding domain (RBD), and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. [provided by RefSeq, Jul 2008]

Uniprot Description

FNBP1: May act as a link between RND2 signaling and regulation of the actin cytoskeleton. Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during the late stage of clathrin-mediated endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization via the recruitment of WASL/N-WASP, which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. May be required for the lysosomal retention of FASLG/FASL. A chromosomal aberration involving FNBP1 is found in acute leukemias. Translocation t(9;11)(q34;q23) with MLL. The relatively low incidence of the MLL-FNBP1 fusion protein in acute leukemia may reflect the marginal capacity of this fusion protein to induce cellular transformation. Belongs to the FNBP1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Actin-binding

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytoskeleton; lysosome; cytoplasmic membrane-bound vesicle; plasma membrane; coated pit; cell cortex

Molecular Function: identical protein binding; protein binding; lipid binding

Biological Process: endocytosis